Abstract：Objective To study the changes in levels of cytokines IL-12 and INF-gamma in patients with chronic hepatitis C after culture in vitro of dendritic cell (DC). Methods Thirty-two patients with chronic hepatitis C were recruited as the observation group, and 30 healthy subjects undergoing physical examination as the control group, 50 ml of anticlotting blood was taken separately. After culture of DC in vitro, 5 ml of supernatant fluid were returned for examination. Using ELISA kit, the cytokines IL-12, INF-gamma level changes before and after culture in the two groups were examined. Results In the observation group, before the experiment, serum cytokine IL-12 was (38.46?4.97) ng/L and IFN - gamma was (15.27?2.67) pg/ml; in the control group, the cytokines were (61.10?4.38) ng/L and (27.24?27.24) pg/ml, respectively; the difference between the two groups was statistically significant (P<0.01). In the observation group, after culture of DC, IL-12 in the supernatant fluid was (337.91?13.35) ng/L, IFN-gamma was (6076.72?403.43) pg/ml; in the control group, the cytokines were (661.44?52.99) ng/L and (10 449.00?626.54) pg/ml, respectively; the difference between the two groups was statistically significant (P<0.01). Conclusions After in vitro culture of DC, secretion of IL-12, and the INF-gamma is obviously higher than that before culture, suggesting that raising the level of cytokines induced by DC immune cells can be used as a new auxiliary therapy in the treatment of chronic hepatitis C.
徐 丽,刘积平,吴晓燕,李修范,杨乐清,闫慧梅,崔宪影. 慢性丙型肝炎患者树突状细胞体外培养后IL-12、INF-γ水平变化[J]. , 2014, 25(1): 37-38.
XU Li,LIU Jiping,WU Xiaoyan,LI Xiufan,YANG Leqing,YAN Huimei,and CUI Xianying. Changes in levels of IL-12 and INF-γafter culture in vitro of dendritic cells of patients with chronic hepatitis C. , 2014, 25(1): 37-38.
Wang Q C, Feng Z H, Zhou Y X, et al.Induction of hepatitis C virus-specific cytotoxic T and B cell responses by dendritic cells expressing a modified antigen targeting receptor[J].World J Gastroenterol, 2005, 11(4):557-560.
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Wang Q C, Feng Z H, Zhou Y X, et al.Induction of hepatitis C virus-specific cytotoxic T and B cell responses by dendritic cells expressing a modified antigen targeting receptor[J].World J Gastroenterol, 2005, 11(4):557-560.
Zammit D J, Lefrancois L.Dendritic cell- T cell interactions in the generation and maintenance of CD+8 T cell memory[J].Microbes Infect, 2006, 8(4):1108-1115.
2014, Vol. 25 
