钛表面TiO<sub>2</sub>纳米管抗菌覆膜药物释放动力学表征及其抗菌活性体外研究

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摘要目的明确钛表面TiO₂纳米管抗菌覆膜药物释放动力学特征,并通过体外试验测试其抗菌活性。方法电化学氧化法在钛金属表面构建TiO₂纳米管表层,利用冻干法加载庆大霉素,在磷酸盐缓冲液中进行药物释放动力学研究。选择标准表皮葡萄球菌菌株,分别在负载和未负载药物纳米管及纯钛3种钛表面进行培养,通过观察细菌粘附和活性菌落存活情况进行表面抗菌活性对比研究。结果 NTS-G庆大霉素药物释放可分为两部分:初始突发释放和后期缓慢释放,初始释放浓度为51.50 μg/mL,第6 h为73.13 μg/ml,大多数负载的庆大霉素大约在9 h内释放。经过一个爆发性初始释放后,庆大霉素从纳米管释放呈现一个平台期,释放量几乎保持不变,维持在89.10 μg/ml水平。3种钛表面体外抗菌活性检测发现,TiO₂纳米管抗菌覆膜假体表面死亡细菌菌落显著增加(P<0.05)。结论钛表面TiO₂纳米管抗菌覆膜为人工关节相关感染预防提供了新途径,骨科植入材料表面纳米管抗生素覆膜具有广泛的应用潜力。

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	冯明光
	刘忠堂
	王立强
	徐丽
	杨海涛
	王健
	王海洋
	秦士新

关键词 ： TiO₂纳米管, 庆大霉素, 药物释放动力学, 表皮葡萄球菌, 抗菌活性

Abstract：Objective To determine the release kinetics of gentamicin from the TiO₂ nanotubes(TNS-G), and to detect its antibacterial activity in vitro. Methods TiO₂ nanotubes (NTS) were fabricated on the titanium surface by electrochemical anodization. These nanotubes were loaded with gentamicin using a lyophilization method and vacuum drying, and its pharmacokinetics was detected in phosphate buffer. Staphylococcus aureus was used to study the antibacterial properties of the NTS-G. There were three study groups: the commercially pure titanium(Cp-Ti)group, the NTS group, and the NTS-G group.We compared the antibacterial efficacy with each other by bacterial adhesion and colony counting in bacterial culture. Results Drug release of NTS-G could be divided into two parts: initial burst release and relatively slow release.The fast initial concentration was 51.50 μg/ml , and 73.13 μg/ml at the 6 th hours. Most of load gentamicin was released within approximately 9 hours.In the second phase, different drug release kinetics were observed, with very slow and linearly increasing cumulative release over a period of 90 h. The drug concentration was maintained at about 89.10 μg/ml. We found that NTS-G could significantly inhibit bacterial adhesion and bioflm formation, compared with Cp-Ti or NTS, (P<0.05). Conclusions Titanium dioxide nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specifc site, thereby providing a new strategy to prevent implant-associated infections.

Key words： TiO₂ nanotubes gentamicin Drug-release kinetics Staphylococcus epidermidis antibacterial activity

收稿日期: 2015-04-22

ZTFLH:

R68

基金资助:国家自然科学基金(81171738);上海市卫生局(2011201)

通讯作者: 刘忠堂,E-mail:surgeon_liu@163.com E-mail: mingguang-feng@126

作者简介: 冯明光,硕士,副主任医师

引用本文:

冯明光,刘忠堂,王立强,徐丽,杨海涛,王健,王海洋,秦士新. 钛表面TiO₂纳米管抗菌覆膜药物释放动力学表征及其抗菌活性体外研究[J]. 武警医学, 2015, 26(11): 1129-1133. FENG Mingguang,LIU Zhongtang, WANG Liqiang, XU Li, YANG Haitao, WANG Jian, WANG Haiyang,and QING Shixin. Drug-release kinetics characterization of gentamicin from TiO₂ nanotubes and its antibacterial activity in vitro. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(11): 1129-1133.

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http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2015/V26/I11/1129

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