Abstract：Objective To develop a noninvasive, quick and reliable method for the identification of Klebsiella pneumonia by detecting its volatile compounds (VCs) using photoacoustic spectroscopy (PAS).Methods Photoacoustic spectroscopy trace analyzer (PASTA) was used to conduct the analysis of bacterial volatile compounds (VCs), such as the volatile metabolites of Klebsiella pneumonia, Acinetobacter baumannii, Escherichia coli and Pseudomonas aeruginosa. The bacteria were cultured at 35 ℃ and measurement of VCs was performed after 24 hours. After that, the difference in the PAS of VCs between Klebsiella pneumonia, blank, Acinetobacter baumannii, Escherichia coli and Pseudomonas aeruginosa was observed.Results The PAS of VCs was basically similar between the same type of bacteria or between the blank groups, indicating that the adopted PASTA was of good performance and the experiment could be easily repeated. Obvious difference in the PAS of VCs was observed between Klebsiella pneumonia and the blank groups, suggesting that new VCs were generated by Klebsiella pneumonia. Besides, the PAS of VCs showed obvious peaks of Klebsiella pneumonia at wave 1142 and 1160 while the PAS of VCs of the other three types of bacteria was smooth at these two wave numbers.Conclusions Identification of Klebsiella pneumonia could be conducted by PAS. The characteristic peak may be located at wave 1142 and 1160.
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