Comparison of profiles between c9orf72-mutant amyotrophic lateral sclerosis and CHMP2B amyotrophic lateral sclerosis based on bioinformatics analysis
LIN Qipeng1, ZHU Qian1,and MA Liwei2
1.Sichuan Provincial Corps Hospital of Chinese people’s Armed Police Force,Leshan 614000,China; 2.Health Bureau of the Logistic Department of Chinese People’s Armed Police Force,Beijing 100081,China
Abstract：Objective To compare the difference of gene expressions between C9orf72 mutation ALS and CHMP2B mutation ALS.Methods Datasets (GSE68605 and GSE19332) were acquired from the gene expression omnibus (GEO) database. R software (version 3.5.0), Cytoscape 3.6.1 software and online tool DAVID and STRING were used for data analysis.Results A total of eleven independent samples (eight samples of C9orf72 mutation ALS and three samples of CHMP2B mutation ALS) were analyzed. Thirteen differentially expressed genes (DEGs) were identified in the cervical spinal cord motor neurons of the two distinct ALS variants. It was found that only CALM2, CALM1, CALM3 and RYR2 were classified during GO and KEEG functional enrichment, and that CALM1, CALM2 and CALM3 were outstanding in the Hub protein analysis.Conclusions The expression of CALM genes is up-regulated in the C9orf72 mutation ALS. Calmodulin might be a potential biomarker for ALS diagnosis and effective target for ALS treatment.
林启鹏,朱茜,马立伟. 两种肌萎缩侧索硬化基因表达谱差异性比较[J]. 武警医学, 2019, 30(2): 124-127.
LIN Qipeng, ZHU Qian,and MA Liwei. Comparison of profiles between c9orf72-mutant amyotrophic lateral sclerosis and CHMP2B amyotrophic lateral sclerosis based on bioinformatics analysis. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(2): 124-127.
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2019, Vol. 30 
