A mouse model of premature atherosclerosis in systemic lupus erythematosus
GUO Xiaoling1, ZHU Chunhong2
1.Department of medicine,Sichuan Provincial Corps Hospital,Chinese People’s Armed Police Force,Chengdu 610041,China; 2.Beijing Railway Center Health and Epidemic Prevention Station Huicheng Gate Clinic,Beijing 100038,China
Abstract：Objective To explore a mouse model of premature atherosclerosis in systemic lupus erythematosus (SLE). Methods Twelve-week-old ApoE-/- mice were randomly divided into the model group and control group.The mouse model was injected with pristane intraperitoneally while the control group was injected with normal saline. After 8 weeks of high fat diet (HFD), all the animals were sacrificed, and the levels of creatinine, urea nitrogen, total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein-cholesterol (HDL-C) were detected using some commercial kits. Hematoxylin (HE) staining was used to observe the morphological changes of kidney tissue. Results Compared with the control group, TC, TG, and LDL-C were increased from (6.52±3.40)mmol/L, (0.49±0.14)mmol/L and(0.30±0.30)mmol/L to (17.53±2.32) mmol/L,(1.03±0.21) mmol/L and (1.25±0.30) mmol/L respectively (P＜0.01), while the level of HDL-C was down from (1.66±0.17)mmol/L to (0.79±0.17)mmol/L (P＜0.01).The levels of creatinine and urea nitrogen were increased significantly. Meanwhile, the renal pathological damage was very severe in the model group. Conclusions A mouse model of premature atherosclerosis in SLE can be established through the intraperitoneal injection with pristane in ApoE-/- mice, which will contribute to the treatment and prevention of premature atherosclerosis in SLE.
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2019, Vol. 30 
