Effects of methane-rich saline on liver mitochondria
ZHU Kaimin1, SHEN Meihua2, WANG Chengwei2, GAO Jing3, CHEN Yan2, and YANG Tao1
1.Department of Anesthesia,the Second Military Medical University,Shanghai 200433,China; 2.Intensive Care Unit,Shanghai Municipal Corps Hospital,Chinese People’s Armed Police Force,Shanghai 201103,China; 3.Institute of Mitochondrial Biology and Medicine, School of Life Science and Technology,Xi’an Jiaotong University,Xi’an 710049,China
Abstract：Objective To study the effect of methane on liver mitochondria in a mouse model of liver ischemia reperfusion.Methods C57 mice were randomly divided into the blank control group (SHAM group n=6), ischemia-reperfusion group ( IR group n=6 ) and methane-rich saline treatment group (IR+CH4 group n=6). The 70% liver ischemia-reperfusion model was adopted. Methane-rich saline 10ml/kg was injected intraperitoneally before reperfusion in the IR+CH4 group. Samples were taken to detect oxidation, antioxidation and mitochondrium related indexes.Results Methane-rich saline increased SOD(t=3.480, P=0.0037)/(GSHt=2.687, P=0.0177) levels but decreased MDA(t=2.771, P=0.015)/ROS(t=3.154, P=0.0083) levels. In the IR group, the expressions of mitochondrial fusion proteins Mfn1(q=7.57,P<0.05) and OPA1(q=6.41,P<0.05) decreased while the expression of mitogen DLP1(q=3.718,P<0.05) increased. PINK1 expression was up-regulated in the IR group (q=4.606,P<0.05)and down-regulated in the IR+CH4 group(q=3.922,P<0.05).Conclusions Methane-rich saline can improve the body’s antioxidant capacity, promote mitochondrial fusion, reduce mitochondrion, and boost the recovery of mitochondrial function.
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2019, Vol. 30 
