Ring finger protein187 promotes the malignancy of glioma cells through pyrolytic pathway
DONG Junqiang1, PEI Meijuan2, HUANG Shengxuan3, LI Xuewen4, TENG Chuan5
1. Department of Neurosurgery, 2. Department of Neurology, 4. Department of Military General Practice, Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin 300162, China; 3. Department of Neurosurgery, Sanming First Hospital Affiliated to Fujian Medical University, Sanming 365000, China; 5. The Second Department of Oncology, the First Hospital of Zibo, Zibo 255299, China
Abstract：Objective To investigate the effect of RNF187 overexpression on human glioma cell U87 and the possible mechanism. Methods Glioma cell U87 was cultured in vitro and transfected with overexpressed RNF187 into U87 cells. The effects of RNF187 overexpression on the growth, proliferation, cell stemness, invasion and metastasis of tumor cells in vitro were analyzed. Finally, the relationship between RNF187 and pyroptosis was studied by detecting the expressions of RNF187 and markers of pyroptosis[inflammatory body 3 (NLRP3), apoptosis associated speck like protein (ASC), GSDMD and GSDME]. Results CCK-8, cell cloning and Transwell experiments confirmed that the morphological swelling of U87 cells increased significantly after overexpression of RNF187, and the cells' ability of proliferation and invasion was significantly enhanced compared with the control group. RT-PCR and Western blot showed that the expression level of RNF187 in glioma cell line was higher than that in normal brain cells. The expression levels of NLRP3, ASC, caspase-1, GSDMD and GSDME in RNF187 the overexpression group were significantly higher than those of the control group (P＜0.05). Conclusions The higher expression of RNF187 in glioma cells can increase the proliferation and invasion of glioma cells, possibly by promoting pyroptosis.
Trejo-Solís C, Serrano-Garcia N, Escamilla-Ramírez Á, et al. Autophagic and apoptotic pathways as targets for chemotherapy in glioblastoma[J]. Int J Mol Sci, 2018,19(12):3773.
[3]
Song H, Wang Y, Shi C, et al. SH3KBP1 promotes glioblastoma tumorigenesis by activating EGFR signaling[J]. Front Oncol, 2021,10:583984.
Wan W B, Wu K, Peng K, et al. High level of RNF187 contributes to the progression and drug resistance of osteosarcoma[J]. J Cancer, 2020,11(6):1351-1358.
[6]
Fu Z, Yu W, Wang H, et al. Overexpression of RNF187 induces cell EMT and apoptosis resistance in NSCLC[J]. J Cell Physiol, 2019,234(8):14161-14169.
[7]
Zhou M, Niu C, Jia L, et al. The value of MGMT promote methylation and IDH-1 mutation on diagnosis of pseudoprogression in patients with high-grade glioma: a meta-analysis[J]. Bull Sch Med Univ Md, 2019,98(50):e18194.
[8]
Lapointe S, Perry A, Butowski NA. primary brain tumours in adults[J]. Lancet, 2018,392(10145):432-446.
[9]
Senft D, Qi J, Ronai ZA. Ubiquitin ligases in oncogenic transformation and cancer therapy[J]. Nat Rev Cancer, 2018,18(2):69-88.
[10]
Qi S M, Cheng G, Cheng X D, et al. Targeting USP7-mediated deubiquitination of MDM2/MDMX-p53 pathway for cancer therapy: are we there yet? [J]. Front Cell Dev Biol, 2020,8:233.
[11]
Wenzel E S, Singh A T K. Cell-cycle checkpoints and aneuploidy on the path to cancer[J]. In Vivo, 2018,32(1):1-5.
[12]
Wang X, Zhang Q, Wang Y, et al. Clinical significance of ubiquitin specific protease 7 (USP7) in predicting prognosis of hepatocellular carcinoma and its functional mechanisms[J]. Med Sci Monit, 2018,24:1742-1750.
[13]
Wang Y, Yin B, Li D, et al. GSDME mediates caspase-3-dependent pyroptosis in gastric cancer[J]. Biochem Biophys Res Commun, 2018,495(1):1418-1425.
Gong X, Du D, Deng Y, et al. The structure and regulation of the E3 ubiquitin ligase HUWE1 and its biological functions in cancer[J]. Invest New Drugs, 2020, 38(2):515-524.
[16]
Lopez-Castejon G. Control of the inflammasome by the ubiquitin system[J]. FEBS J, 2020,287(1):11-26.
[17]
Huang L, Luo R, Li J, et al. β-catenin promotes NLRP3 inflammasome activation via increasing the association between NLRP3 and ASC[J]. Mol Immunol, 2020,121:186-194.
[18]
Song S, Qiu D, Luo F, et al. Knockdown of NLRP3 alleviates high glucose or TGFB1-induced EMT in human renal tubular cells[J]. J Mol Endocrinol, 2018,61(3):101-113.
[19]
Shi J, Gao W, Shao F. Pyroptosis: gasdermin-mediated programmed necrotic cell death[J]. Trends Biochem Sci, 2017,42(4):245-254.
[20]
Zhang D, Qian J, Zhang P, et al. Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro[J]. J Neurosci Res, 2019,97(6):645-660.
2021, Vol. 32 
