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| Evaluation of eliminating lipid interference in biochemical examination using dilution method |
| ZHANG Yunxiu,SHI Yuying,SHENG Huiguang, and WANG Peichang. Clinical Laboratory, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053,China |
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Abstract To compare the difference in the effect of eliminating lipid interference in biochemical examination between dilution with deionized water and high-speed centrifugation. MethodsThe appropriate dilution with deionized water by triacylglycerol nterference test was selected. Dilution with deionized water and high-speed centrifugation (13000 r/m, 10 min) were compared in the effect of eliminating lipid interference in biochemical examination and analyzed using paired T-test. ResultsALT, AST,CK, CKMB, CREA, UREA, GLU, AMY, and LPS results by dilution with deionized water were consistent with those by high-speed centrifugation. The difference was not statistically significant. ConclusionsDilution with deionized water can eliminate the impact of lipids and provide clinicians with accurate biochemical test results.
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石凌波, 史惠群.利用高速离心法消除脂血对生化测定的干扰［J ］.检验医学, 2004,19(2): 138-140.［2］张帆.高速离心对临床常规生化项目测定结果的影响［J］.国际检验医学杂志，2010,31(8):887-888.［3］熊俊, 石文静, 闪全忠. 磷钨酸-镁-PEG 法消除脂血干扰的方法学评估［J］. 现代检验医学杂志, 2008,23(1)：50-52.［4］Clinical and Laboratory Standards Institute．Interference testing in clinical chemistry［S］．2nd ed．EP7-A2，CLSI，2005．［5］徐建华，何敏，柯培锋，等.CLSI EP7-A2文件在临床化学分析干扰试验中的应用评价［J］.检验医学,2010,25(12):971-974.［6］王景顺，田浩，陈鑫.萃取法去除脂血对ISE间接测定的影响［J］. 医学信息,2006, 19(1):129-130.［7］刘俊,黄文红,付波.三种消除血浆高脂质浑浊方法对酶类检测结果的影响［J］.华南国防医学杂志，2010，24(1)：42-43.［8］张敏, 吕礼应, 刘万利, 等. 不同程度的脂血和黄疸对酶法测定血清钾干扰的分析［J］ . 蚌埠医学院学报, 2004, 29( 5) : 465-467.
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| ［1］ |
石凌波, 史惠群.利用高速离心法消除脂血对生化测定的干扰［J ］.检验医学, 2004,19(2): 138-140.［2］张帆.高速离心对临床常规生化项目测定结果的影响［J］.国际检验医学杂志，2010,31(8):887-888.［3］熊俊, 石文静, 闪全忠. 磷钨酸-镁-PEG 法消除脂血干扰的方法学评估［J］. 现代检验医学杂志, 2008,23(1)：50-52.［4］Clinical and Laboratory Standards Institute．Interference testing in clinical chemistry［S］．2nd ed．EP7-A2，CLSI，2005．［5］徐建华，何敏，柯培锋，等.CLSI EP7-A2文件在临床化学分析干扰试验中的应用评价［J］.检验医学,2010,25(12):971-974.［6］王景顺，田浩，陈鑫.萃取法去除脂血对ISE间接测定的影响［J］. 医学信息,2006, 19(1):129-130.［7］刘俊,黄文红,付波.三种消除血浆高脂质浑浊方法对酶类检测结果的影响［J］.华南国防医学杂志，2010，24(1)：42-43.［8］张敏, 吕礼应, 刘万利, 等. 不同程度的脂血和黄疸对酶法测定血清钾干扰的分析［J］ . 蚌埠医学院学报, 2004, 29( 5) : 465-467.
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