重庆地区儿童急性腹泻相关博卡病毒的基因类型及其流行特征调查

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Abstract Objective To investigate the prevalence, genotypes and epidemic characteristics of human bocavirus associated with acute pediatric diarrhea in Chongqing area. Methods Pediatric patients with acute diarrhea were recruited and stool samples were collected in Children’s Hospital of Chongqing Medical University during 2011. Real-time PCR and nested PCR were performed to determine the presence of HBoV and their genotypes. Results A total of 504 stool specimens were collected, from which 49 HBoV positive samples were identified, with a detection rate of 9.72%. A seasonal distribution of HBoV was observed, with a peak (19.72%) in summer. No distribution of age or gender specific was obtained for the HBoV positive detection. Based on Blast and MEGA analysis, 25 HBoV1, 23 HBoV2 and 1 HBoV3 were determined. All the HBoV2 strains detected belonged to HBoV-2B genotype. Conclusions The detection rate of HBoV has obvious seasonality, with a peak in summer. HBoV1 and HBoV-2B are the major genotypes.

Key words： human bocavirus acute pediatric diarrhea investigation genotype

Received: 04 June 2014

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	ZHAO Jin
	HUANG Dou-Dou
	LI Si-Si
	GUO Chen-Tao
	XU Hong-Mei
	ZHANG Xiao-Ai
	LIU Wei

Cite this article:

ZHAO Jin,HUANG Dou-Dou,LI Si-Si等. Investigation of human bocavirus in pediatric patients with acute diarrhea in Chongqing, China[J]. , 2014, 25(8): 797-799.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2014/V25/I8/797

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