三氧化二砷对内皮细胞增殖和一氧化氮分泌的影响

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Abstract Objective To investigate the effect of As₂O₃ on proliferation of human umbilical endothelial cells, and nitric oxide(NO)release, and to evaluate the value of NO in inhibiting restenosis. Methods Cultured cells were divided into seven groups, one as control group, the other six groups were incubated with As₂O₃ at deferent concentrations(0.01, 0.1, 0.5, 1, 5, 10 μmol/L).Effect of As₂O₃ on proliferation of human umbilical endothelial cells(ECV-304) was detected by 3H-TdR assay at 24 h, 48 h, 72 h and nitric oxide (NO) production was elected. Results When the concentration of As₂O₃ was lower than 1 μmol/L, it had no effect on endothelial cells proliferation.When the concentration of As₂O₃ was lower than 0.1 μmol/L, it had no effect on NO production.NO production was reduced in dose dependent manner, when endothelial cells were incubated in As₂O₃ within the concentration range of 0.5~10 μmol/L. Conclusions When the concentration of As₂O₃ is lower than 0.1 μmol/L, it has no effect on endothelial cells proliferation and NO production. As₂O₃ within the concentration range of 0.1~1 μmol/L can reduce NO production, but has no effect on endothelial cells proliferation. As₂O₃ at low concentration may be used to reduce restenosis.

Key words： arsenic trioxide endothelial cells proliferation nitric oxide

Received: 23 July 2014

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	WANG Xueqing
	CHEN Yucheng
	ZENG Zhi

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WANG Xueqing,CHEN Yucheng,ZENG Zhi. Effect of arsenic trioxide on endothelial cells proliferation and nitric oxide production[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(1): 29-31.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I1/29

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