高效液相色谱法测定奥扎格雷钠脂质体的包封率

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (547 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To establish a high performance liquid chromatography (HPLC) method for the encapsulation efficiency of ozagrel hydrochloride liposome. Methods The HPLC was used for determination. A C18 column was used with the mobile phase of 0.05 mol/L potassium dihydrogen phosphate solution (pH=7.0) - acetonitrile - methanol (80∶15∶5) at the detection wavelength of 274 nm. Results The calibration curve was linear in the concentration range of 10-100 μg/ml, r=0.9997. The inter- and intra- day RSD was less than 0.5 %. The method recoveries were (100.2±0.42) %. The average entrapment efficiency of tartrate indoquinoline liposome was (94.89±0.44)%. Conclusions This method is simple,accurate and can be used to determine the encapsulation efficiency of ozagrel hydroehloride in ozagrel hydrochloride liposome.

Key words： ozagrel hydroehloride liposome encapsulation efficiency HPLC

Received: 15 November 2014

ZTFLH:

R284.2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LIU Qing
	LIU Lifeng
	SUN Changyou
	Li Huifang

Cite this article:

LIU Qing,LIU Lifeng,SUN Changyou等. Encapsulation efficiency of ozagrel hydroehloride liposome by HPLC[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(6): 584-586.

URL:

http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I6/584

[1]	刘洋. 奥扎格雷治疗脑血栓形成的临床研究[J]. 中国实用医药,2014,9(34): 142-144.
[1]	刘洋. 奥扎格雷治疗脑血栓形成的临床研究[J]. 中国实用医药,2014,9(34): 142-144.
[2]	安小兰. 奥扎格雷治疗脑梗死临床疗效观察[J]. 医学理论与实践,2013,26(21): 2844-2845.
[2]	安小兰. 奥扎格雷治疗脑梗死临床疗效观察[J]. 医学理论与实践,2013,26(21): 2844-2845.
[3]	WS1-(X-095)-2005Z,国家食品药品监督管理局国家药品标准[S].
[3]	WS1-(X-095)-2005Z,国家食品药品监督管理局国家药品标准[S].
[4]	张勇,陈玉威,高桂花.内标法测定奥扎格雷钠颗粒的含量[J].实用药物与临床,2013,1(6): 517-519.
[4]	张勇,陈玉威,高桂花.内标法测定奥扎格雷钠颗粒的含量[J].实用药物与临床,2013,1(6): 517-519.
[5]	沈晓霞.HPLC法测定注射用奥扎格雷钠的含量[J].中国药师,2007,10(4): 348-350.
[6]	徐小梅,程庆兵.HPLC法测定注射用奥扎格雷钠的含量[J].安徽卫生职业技术学院学报,2013,12(5): 84-85.
[5]	沈晓霞.HPLC法测定注射用奥扎格雷钠的含量[J].中国药师,2007,10(4): 348-350.
[6]	徐小梅,程庆兵.HPLC法测定注射用奥扎格雷钠的含量[J].安徽卫生职业技术学院学报,2013,12(5): 84-85.
[7]	朱平,李娜,王志敏.高效液相色谱法测定奥扎格雷钠注射液中有关物质[J].山西医科大学学报,2007,38(11): 1010-1012.
[8]	于明,张迪,李沫,等.奥扎格雷钠氯化钠注射液中特定杂质的研究[J].中国药师,2012,14(10): 1421-1423.
[7]	朱平,李娜,王志敏.高效液相色谱法测定奥扎格雷钠注射液中有关物质[J].山西医科大学学报,2007,38(11): 1010-1012.
[8]	于明,张迪,李沫,等.奥扎格雷钠氯化钠注射液中特定杂质的研究[J].中国药师,2012,14(10): 1421-1423.
[9]	孟玉玲,孟祥军,葛欣.HPLC法测定奥扎格雷钠注射液的含量[J].中国现代药物应用,2010,4(21): 32-33.
[9]	孟玉玲,孟祥军,葛欣.HPLC法测定奥扎格雷钠注射液的含量[J].中国现代药物应用,2010,4(21): 32-33.