survivin在肝癌细胞株Hep G<sub>2</sub>表达与化疗药物敏感性的关系

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Abstract Objective To investigate the relationship between survivin and chemotherapeutic sensitivity in hepatocellular carcinoma cells. Methods By the method of MTT, the sensitivity of Hep G₂ was tested to be 1.0 μg/ml cisplatin, 1 μmol/L doxorubicin and 1.0 μg/ml gemcitabine. Western blot was used to detect the expression of survivin protein at various time points after the drugs intervention. Results Cultured for 72 hours, 10-20% of Hep G₂ cells were still alive(2.039±0.05 vs 1.258±0.05;1.843±0.02 vs 1.258±0.05;1.924±0.05 vs 1.551±0.04;1.884±0.11 vs 1.551±0.04) ,P<0.05. After cisplatin and gemcitabine intervention for 48 hours, the expression of survivin was elevated (P<0.05). After adriamycin treatment for 48 hours, the expression of survivin protein had no significant change(1.729±0.11 vs 1.518±0.02;1.667±0.12 vs 1.518±0.02). Conclusions In Hep G₂, the high expression of survivin is associated with the chemosensitivities of parts of drugs.

Key words： hepatoma survivin cisplatin gemcitabine doxorubicin

Received: 10 December 2014

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	LIANG Rui
	MENG Hontao
	DUAN Wei
	WANG Jialin
	LI Zhanning
	XU Wenhuan

Cite this article:

LIANG Rui,MENG Hontao,DUAN Wei等. Relationship between changes of survivin expression and chemotherapeutic sensitivity in Hep G₂[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(8): 812-814.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I8/812

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