骨桥蛋白对RANKL诱导破骨细胞分化的影响

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Abstract Objective To study the effect of osteopontin (OPN) on differentiation of osteoclasts (OC) by RANKL in vitro. Methods Mouse osteoclasts precursor cell line RAW264.7 was cultured and induced to form OC, and was added with osteopontin (OPN). Then, the proliferation and differentiation of OC were detected by MTT assay and TRAP staining. Furthermore, RT-PCR was used to measure the gene expression of RANK. Results The model of OC in vitro was established successfully, accompanied by the significant increase of proliferation and differentiation of OC (P<0.05). Meanwhile, OPN promoted the expression of RANK mRNA in OC obviously, which could be increased 2.67 times. Conclusions OPN affects the differentiation of RAW264.7 cells to form OC via RANK/ RANKL pathway, which may provide a new target for the prevention and treatment of osteoporosis and bone-related diseases.

Key words： osteopontin RAW264.7 cells osteoclasts differentiation

Received: 01 September 2015

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	Articles by authors
	WANG Zengtian
	XING Jie
	and LI Tan

Cite this article:

WANG Zengtian,XING Jie,and LI Tan. Effect of osteopontin on differentiation of osteoclasts induced by RANKL[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(12): 1221-1223.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I12/1221

[1]	Udagawa N, Koide M, Horibe K, et al. Osteoclast-mediated demineralization of biomineral[J]. Clin Calcium, 2014,24(2):215-223.
[1]	Udagawa N, Koide M, Horibe K, et al. Osteoclast-mediated demineralization of biomineral[J]. Clin Calcium, 2014,24(2):215-223.
[2]	Fodor D, Bondor C, Albu A, et al. The value of osteopontin in the assessment of bone mineral density status in postmenopausal women[J]. J Investig Med, 2013,61(1):15-21.
[2]	Fodor D, Bondor C, Albu A, et al. The value of osteopontin in the assessment of bone mineral density status in postmenopausal women[J]. J Investig Med, 2013,61(1):15-21.
[3]	Takahashi N, Udagawa N, Kobayashi Y, et al. Generation of osteoclasts in vitro, and assay of osteoclast activity[J]. Methods Mol Med, 2007,135(1): 285-301.
[3]	Takahashi N, Udagawa N, Kobayashi Y, et al. Generation of osteoclasts in vitro, and assay of osteoclast activity[J]. Methods Mol Med, 2007,135(1): 285-301.
[4]	Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays[J]. J Immunol Methods, 1983, 65 (1-2):55-63.
[4]	Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays[J]. J Immunol Methods, 1983, 65 (1-2):55-63.
[5]	Ishii M, Saeki Y. Osteoclast cell fusion:mechanisms and molecules[J]. Mod Rheumatol, 2008,18(3):220-227.
[5]	Ishii M, Saeki Y. Osteoclast cell fusion:mechanisms and molecules[J]. Mod Rheumatol, 2008,18(3):220-227.
[6]	Hunter GK. Role of osteopontin in modulation of hydroxyapatite formation[J]. Calcif Tissue Int, 2013,93(4):348-354.
[6]	Hunter GK. Role of osteopontin in modulation of hydroxyapatite formation[J]. Calcif Tissue Int, 2013,93(4):348-354.
[7]	Ishijima M, Tsuji K, Rittling S R, et al. Role of osteopontin in mediating mechanical stress effect on bone[J]. J Endocrinol, 2007,193(2):235-243.
[7]	Ishijima M, Tsuji K, Rittling S R, et al. Role of osteopontin in mediating mechanical stress effect on bone[J]. J Endocrinol, 2007,193(2):235-243.
[8]	Walker C G, Dangaria S, Ito Y, et al. Osteopontin is required for unloading-induced osteoclast recruitment and modulation of RANKL expression during tooth drift-associated bone remodeling, but not for super-eruption[J]. Bone, 2010,47(6):1020-1029.
[8]	Walker C G, Dangaria S, Ito Y, et al. Osteopontin is required for unloading-induced osteoclast recruitment and modulation of RANKL expression during tooth drift-associated bone remodeling, but not for super-eruption[J]. Bone, 2010,47(6):1020-1029.
[9]	Hanada R, Hanada T, Penninger J M. Physiology and pathophysiology of the RANKL /RANK system[J]. Biol Chem, 2010,391(12):1365-1370.
[10]	Arriero Mdel M, Ramis J M, Perelló J, et al. Inositol hexakisphosphate inhibits osteoclastogenesis on RAW 264. 7 cells and human primary osteoclasts[J]. PLoS ONE, 2012,7 (8):e43187.
[9]	Hanada R, Hanada T, Penninger J M. Physiology and pathophysiology of the RANKL /RANK system[J]. Biol Chem, 2010,391(12):1365-1370.
[11]	Rhim E M, Ahn S J, Kim J Y, et al. Cryopreservation induces macrophage colony stimulating factor from human periodontal ligament cells in vitro[J]. Cryobiology, 2013,67(2):156-162.
[10]	Arriero Mdel M, Ramis J M, Perelló J, et al. Inositol hexakisphosphate inhibits osteoclastogenesis on RAW 264. 7 cells and human primary osteoclasts[J]. PLoS ONE, 2012,7 (8):e43187.
[11]	Rhim E M, Ahn S J, Kim J Y, et al. Cryopreservation induces macrophage colony stimulating factor from human periodontal ligament cells in vitro[J]. Cryobiology, 2013,67(2):156-162.