大鼠心肌缺血-再灌注损伤miRNA-214介导电针预处理的保护作用

Abstract
Figure/Table
References
Related Citation (7)

Download: PDF (842 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To evaluate whether miRNA-214 mechanism was involved in the cardioprotection by electroacupuncture pretreatment of myocardial ischemia/ reperfusion injury in rats. Methods 24 male SD rats were divided into 4 groups randomly (n=6): control group (sham group), sham + electric acupuncture group (Sham+EA) group, ischemia/reperfusion group (I/R group), ischemia/reperfusion + electric acupuncture group (I/R + EA group). Rats in group sham+EA and I/R+EA received electroacupuncture stimulation 30 min per day for 3 days before myocardial ischemia/reperfusion injury. Rats in group I/R and I/R+EA received left anterior descending (LAD) occlusion for 40min and reperfusion for 180 min. The indices of the cardiac function, myocardial infarct size , LDH and CK activities as well as expression of miRNA-214 were measured. Results All the indices of the cardiac function were not significantly different between the sham and the sham+EA group. Compared with the sham group, all the indices of the cardiac function decreased significantly in I/R group (P<0.05). I/R+EA group showed that all the indices of the cardiac function increased, compared with I/R group. Myocardial infarct size in I/R+EA group significantly decreased, compared with I/R group. The activity of LDH and CK in of I/R group significantly increased, compared with I/R+EA group (P<0.05 or P<0.01). The expression of miRNA-214 in sham, sham+EA, I/R and I/R+EA group were 1, 1.6, 1.75 and 2.82, separately. The expression of miRNA-214 in the I/R group was higher than in sham group. Electroacupuncture pretreatment increased miR - 214 expression in sham + EA group and the I/R + EA group. Conclusions Electroacupuncture pretreatment can protect the heart against myocardial ischemia/ reperfusion injury and can promote the expression of miRNA-214 in rats.

Key words： I/R injury miRNA-214 electroacupuncture protective effect

Received: 06 April 2015

ZTFLH:

R541.9

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LIU Peiyu
	TIAN Yi
	BAO Manxi
	WANG Yue
	and CUI Fen

Cite this article:

LIU Peiyu,TIAN Yi,BAO Manxi等. miRNA-214 was involved in cardioprotection by electroacupuncture pretreatment of myocardial ischemia/ reperfusion injury in rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(1): 43-47.

URL:

http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I1/43

[1]	Ju Z, Cui H, Guo X, et al. Molecular mechanisms underlying the effects of acupuncture on neuropathic pain[J]. Neural Regen Res,2013,8(25):2350-2359.
[2]	Yu Z, Luo L, Li Y, et al. Different manual manipulations and electrical parameters exert different therapeutic effects of acupuncture[J]. J Tradit Chin Med, 2014,34(6):754-758.
[3]	Inoue M, Nakajima M, Oi Y,et al. The effect of electroacupuncture on tendon repair in a rat Achilles tendon rupture model[J]. Acupunct Med, 2015,33(1):58-64.
[4]	Tjen-A-Looi S C, Guo Z L, Longhurst J C. GABA in nucleus tractus solitarius participates inelectroacupuncture modulation of cardiopulmonary bradycardia reflex[J]. Am J Physiol Regul Integr Comp Physiol,2014,307(11):R1313-1323.
[5]	Yu H J, Zhu J G, Shen P,et al. Electroacupuncture decreases the urinary bladder pressure in patients with acute gastrointestinal injury[J]. Genet Mol Res, 2015,14(1):34-39.
[6]	Liu P Y, Tian Y, Xu S Y. Mediated protective effect of electroacupuncture pretreatment by miRNA-214 on myocardial ischemia/reperfusion injury[J]. J Geriatr Cardiol, 2014,11(4):303-310.
[7]	Toosizadeh N, Lei H, Schwenk M,et al. Does integrative medicine enhance balance in aging adults? Proof of concept for the benefit of electroacupuncture therapy in Parkinson’s disease[J]. Gerontology, 2015, 61(1): 3-14.
[8]	Chung J Y, Yoo D Y, Im W, et al. Electroacupuncture at the Zusanli and Baihui acupoints ameliorates type-2 diabetes-induced reductions in proliferating cells and differentiated neuroblasts in the hippocampal dentate gyrus with increasing brain-derived neurotrophic factor levels[J]. Vet Med Sci,2015,77(2):167-173.
[9]	Siddall H K, Warrell C E, Yellon D M, et al. Ischemia-reperfusion injury and cardioprotection: investigating PTEN, the phosphatase that negatively regulates PI3K, using a congenital model of PTEN haploinsufficiency[J]. Basic Res Cardiol, 2008, 103(6):560-568.
[10]	Ahmed M I, Alam M, Emelianov V U, et al.MiRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway[J]. J Cell Biol, 2014, 207(4): 549-567.
[11]	Takano H, Zou Y, Hasegawa H, et al. Oxidative stress-induced signal transduction pathways in cardiac myocytes: involvement of ROS in heart diseases[J]. Antioxid Redox Signal, 2003, 5(12): 789-794.
[12]	Wang X, Sun J, Fu C, et al.MiRNA-214 regulates osteosarcoma survival and growth by directly targeting phosphatase and tensin homolog[J]. Mol Med Rep, 2014,10(6):3073-3079.
[13]	Yang H, Kong W, He L, et al. MicroRNA expression profiling in humanovarian cancer: miRNA-214 induces cell survival and cisplatin resistance by targeting PTEN[J]. Cancer Res, 2008, 68(2): 425-433.
[14]	Tekin D, Dursun A D, Xi L. Hypoxia inducible factor 1 (hif-1) and cardioprotection[J]. Acta Pharmacol Sin, 2010, 31(9): 1085-1094.
[15]	张苗苗,于海奕,祖凌云,等.miRNA-214 在大鼠心肌缺血-再灌注损伤及缺血后适应中的表达及机制探讨[J].中国分子心脏病学杂志, 2011, 11(2): 108-112.
[16]	Aurora A B, Mahmoud A I, Luo X, et al. MiRNA-214 protects the 43 mouse heart from ischemic injury by controlling Ca(2)(+) overload and cell death[J]. J Clin Invest, 2012, 122(4):1222-1232.
[17]	Langevin H M, Schnyer R, MacPherson H, et al. Manual and electrical needle stimulation in acupuncture research: pitfalls and challenges of heterogeneity[J].J Altern Complement Med,2015,21(3):113-128.
[18]	Hu S, Zhao Z K, Liu R, et al. Electroacupuncture activates enteric glial cells and protects the gut barrier in hemorrhaged rats[J]. World J Gastroenterol, 2015,21(5):1468-1478.
[19]	Aranha M F, Müller C E, Gavio M B. Pain intensity and cervical range of motion in women with myofascial pain treated with acupuncture and electroacupuncture: a double-blinded, randomized clinical trial[J]. Braz J Phys Ther,2015,19(1):34-43.
[20]	Mohammad A S, Mohammad H E, Mohammad B K, et al. Electro-acupuncture Stimulation at Acupoints Reduced the Severity of hypotension during anesthesia in patients undergoing liver transplantation[J]. J Acupunct Meridian Stud, 2012, 5(1):11-14.