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| Effects of acid-elution temperature of hemoglobin F on precision of Kleihauer-Betke test |
| ZHU Hongyuan1, FAN Fei1, CAO Zheng1, CHEN Kexin2, ZENG Jiazi1, XIE Xin1, YANG Lijun3 |
1.Department of Clinical Laboratory,3.Develpoment and Operations Department, Obstetrics and Gynecology Hospital Affiliated to Capital Medical University, Beijing 100026, China;
2. Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Beijing Tongren Eye Center, Capital Medical University, Beijing Ophthalmology & Visual Science Key Lab, Beijing 100730, China |
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Abstract Objective To determine the optimum acid-elution temperature of Kleihauer-Betke (K-B) experiment for hemoglobin F detection and fetomaternal hemorrhage (FMH) evaluation.Methods Both 37 ℃ and 45 ℃ were chosen as the acid-elution temperatures in the K-B experiment, followed by head-to-head staining results comparison.Results When the elution temperature was set at 37 ℃ and specimens were negative, there was no statistically significant difference between the results of K-B experiments (< 1%) and theoretical values. However, with theoretically positive specimens (≥1%), the results of experiments were statistically different from the calculated values (P<0.05). When the elution temperature was set at 45 ℃, there was no significant difference between the K-B test results and their corresponding theoretical values, whether the specimens were positive or. negative.Conclusion When the elution temperature is set at 45 ℃ in the K-B experiment, we can more effectively and accurately distinguish the fetal red blood cells from maternal blood by the staining method. Our work provides helpful laboratory data support for the diagnosis, treatment and prognosis of FMH.
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Received: 16 January 2019
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2019, Vol. 30 
