GDF11对糖尿病小鼠骨髓间充质干细胞细胞凋亡的影响及其信号机制

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Abstract Objective To investigate the effects of GDF11 on apoptosis of BMSCs from diabetic mice and the related mechanism.Methods BMSCs of normal mice (Nor),diabetic mice (DB),DB with GDF11 intervention (GDF11),and DB+GDF11 plus LY294002 (LY) were cultured for 3 days.Trypan blue staining was used to calculate the apoptotic rate,while western blot was used to investigate protein levels of Bax,Bcl-2,phosphorylated (p)-PI3K and p-Akt.Results Compared with Nor,the apoptotic rate of DB was increased [(36.2±3.3)% vs. (3.1±1.1)%,P＜0.05],but GDF11 intervention could reduce the apoptotic rate [(18.9±1.9)% vs. (36.2±3.3)%,P＜0.05],increase Bcl-2/Bax expression[(1.63±0.10) vs. (0.26±0.09);P＜0.05],and activate the PI3K[p-PI3K/PI3K:(0.98±0.08) vs. (0.42±0.11);P＜0.05]/Akt[p-Akt/Akt:(0.94±0.10) vs. (0.32±0.15);P＜0.05]signaling pathway.However,when inhibiting PI3K/Akt signaling pathway,GDF11 had its inhibitory effect on cell apoptosis blunted[apoptotic rate:(41.1±3.9)% vs. (18.9±1.9)%,P＜0.05].Conclusions GDF11 can inhibit the apoptosis of BMSCs from diabetic mice by activating the PI3K/Akt signaling pathway.

Key words： DGF11 diabetes bone-marrow mesenchymal stem cells (BMSCs) cell apoptosis

Received: 27 July 2020

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	Articles by authors
	ZHAI Yu
	WU Fangli
	TIAN Xiaoyu
	LI Shuang
	GUO Shuqin
	ZHU Biao
	ZHAO Lisheng
	GUO Hongyan

Cite this article:

ZHAI Yu,WU Fangli,TIAN Xiaoyu等. Effects of GDF11 on apoptosis of bone-marrow mesenchymal stem cells from diabetic mice and related mechanisms[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(1): 19-21.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2021/V32/I1/19

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