高盐诱导下以 TonEBP/NF-κB 通路为基础的小鼠巨噬细胞表型偏移机制的研究

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Abstract Objective To investigate the possible mechanism of macrophage phenotypic polarization under hypersaline stimulation so as to provide data for future research into the prevention and treatment of salt-sensitive hypertension and other macrophage related cardiovascular diseases.Methods Using Raw264.7 mouse macrophages as the subjects, a TonEBP lentivirus interference stable cell line was constructed. The interference efficiency of TonEBP gene was detected by real-time PCR , and the cell lines of the infected group with the highest interference efficiency were selected for subsequent experiments.The mRNA expression levels of TonEBP、NF-κB 、M1-type and M2-type macrophage phenotypic markers in each group were detected by real-time PCR after twenty hours of corresponding intervention.Western blot was used to detect the protein expression levels of TonEBP、NF-κB、p-NF-κB and p-IKKα/β.Results Stable interfering cell lines of TonEBP-shRNA were constructed, and the TonEBP interfering efficiency was over 70% compared with the control group (P＜0.05).The mRNA expression levels of TonEBP、NF-κB andM1 macrophage phenotypic markers in the HS group were significantly up-regulated,(P＜0.05), while the mRNA expression levels of M2 type macrophage phenotypic markers were significantly down-regulated(P＜0.05).mRNA expression of M1-type macrophage phenotypic markers was significantly down-regulated,(P＜0.05) while mRNA expression of M2-type macrophage phenotypic markers was significantly up-regulated by interfering with the expressions of TonEBP and NF-κB respectively under high salt stimulation(P＜0.05).Compared with the control group, the protein expression levels of TonEBP、p-IKKα/β、NF-κB and p-NF-κB in the HS group were significantly up-regulated(P＜0.05), and the ratio of p-NF-κB to NF-κB was increased(P＜0.05).Compared with the HS group, protein expression levels of p-IKKα/β、NF-κB and NF-κB were significantly decreased after interference with the expression of TonEBP and NF-κB respectively under high salt stimulation(P＜0.05),but difference in the ratio of p-NF-κB to NF-κB was of no statistical significance(P＞0.05).Conclusions Activation of TonEBP/NF-κB signaling pathway is a possible mechanism of Raw264.7 cell shifting to M1 phenotype under high salt stimulation.The regulation of TonEBP/ NF-κB signaling pathway can change the direction in which the phenotypes of Raw264.7 cells are polarized under high salt induction.

Key words： high salt cardiovascular disease macrophages phenotype signaling pathway

Received: 22 January 2021

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	ZHU Zhe
	YANG Guohong
	ZHAO Jihong

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ZHU Zhe,YANG Guohong,ZHAO Jihong. Mechanisms of macrophage phenotype polarization based on the TonEBP/NF-κB pathway under high salt intervention[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(8): 676-680.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2021/V32/I8/676

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