α7烟碱样乙酰胆碱受体激动剂后处理对高胆固醇血症大鼠在体心肌缺血/再灌注损伤的影响

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Abstract Objective To evaluate the effect of postconditioning with α7 nicotinic acetylcholine receptor (α7nAChR) agonist on myocardial protection in hypercholesterolemic rats.Methods A hypercholesterolemic model of rats was induced by means of a eight-week diet containing 2% cholesterol and 0.5% bile salts. Hypercholesterolemic and normal rats were randomly divided into three groups respectively (n=5): control group (group HC and NC), ischemia/reperfusion injury group (group HI and NI), and PNU282987 postconditioning group (group HPNU and NPNU). Serum levels of total cholesterol (TC), low-density lipoprotein (LDL), creatine kinase MB (CK-MB), cardiac troponin I (cTnI), tumor necrosis factor (TNF) -α and interleukin (IL) -6 were assayed. The size of myocardial infarction was determined by Evans blue and triphenyltetrazolium chloride (TTC) staining.Results Compared with group NC, levels of TC and LDL were elevated significantly in group HC (P<0.05). Compared with group NI, serum levels of CKMB, cTnI, TNF-α, IL-6, and myocardial infarct size were decreased significantly in group NPNU (P<0.05). No significant difference was found between group HI and group HPNU (P>0.05).Conclusions Hypercholesterolemia can aggravate myocardial ischemia/reperfusion injury and eliminate the myocardial protective effect of α7nAChR agonist postconditioning against myocardial ischemia/reperfusion injury.

Key words： ischemia/reperfusion injury hypercholesterolemia cardioprotection

Received: 19 June 2021

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	WEN Chao
	XUE Fushan
	WANG Yuhui
	JIN Jinhua

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WEN Chao,XUE Fushan,WANG Yuhui等. Effect of postconditioning with α7 nicotinic acetylcholine receptor agonist on myocardial ischemia/reperfusion injury in rats with hypercholesterolemia[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(8): 698-701.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2021/V32/I8/698

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