电针足三里对失血性休克海水浸泡损伤延迟静脉复苏大鼠生存率及脏器功能的影响

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Abstract Objective To study the effects of electroacupuncture at Zusanli(ST36) point on the survival rate and organ function of rats with delayed intravenous resuscitation from seawater immersion injury in hemorrhagic shock (HS). Methods 100SPF-grade male SD rats were selected and divided into HS + seawater immersion group (control group) and HS + seawater immersion + electroacupuncture group (electroacupuncture group) according to the random number table method. The rats were rapidly bled via the carotid artery, and the blood loss was 45% of the total blood volume. Immediately after blood loss, the body below the glabella was immersed in artificial seawater at (23±1) ℃ for 30 min. Blood loss and 2 times the amount of blood loss in lactated Ringer’s solution were returned 2 h after seawater immersion. The electroacupuncture group was acupunctured at 0.5 h after immersion with bilateral ST36, and the control group was not acupunctured. The 24 h survival rate and the changes ofCr, CK-MB, ALT, and DAO before blood loss, immediately after immersion, and 2 h, 5 h, and 24 h after immersion were monitored in both groups. Results The 24h survival rate was significantly higher in the electroacupuncture group (90.0%) than in the control group (50.0%,χ²=4.494,P=0.034). The DAO of the electroacupuncture group(9.98±1.00)was significantly lower than that of the control group 2h after immersion (11.99±0.85,t=4.325,P=0.001). At 5 h after immersion, CK-MB(501.00±204.94), ALT(65.38±7.67), and DAO(8.37±1.54) in the electroacupuncture group were significantly lower than those in the control group (1101.38±336.01,t=4.315, P=0.001;97.64±7.90,t=8.286,P=0.000;10.02±0.91,t=2.367,P=0.035). The Cr(19.50±4.11)in the electroacupuncture group was lower than that in the control group 24 h after immersion (24.88±2.42,t=3.191,P=0.008). Conclusions Electroacupuncture of the ST36 can improve the 24 h survival rate of rats with delayed intravenous resuscitation by seawater immersion following hemorrhagic shock, improve the organ function of animals injured by seawater immersion in hemorrhagic shock, and has the effect of anti-shock.

Key words： electroacupuncture Zusanli hemorrhagic shock seawater immersion delayed intravenous resuscitation organ function

Received: 21 April 2022

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	WANG Lili
	HU Sen
	ZHANG Haipai
	TIAN Yumo
	ZHANG Huiping
	GUAN Ling

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WANG Lili,HU Sen,ZHANG Haipai等. Effects of electroacupuncture at ST36 point on the survival rate and organ function of rats with delayed intravenous resuscitation from seawater immersion injury following hemorrhagic shock[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(1): 26-29.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2023/V34/I1/26

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