Effect of gefitinib on growth of human lung cancer cell line A549
JIANG Xuedong1, ZHU Jiang2, LIU Changhao1, QUAN Liguo1, and WANG Guozhu1.
1.Department of Thoracic Surgery,
2.Department of Medical Service, Liaoning Provincial Corps Hospital, Chinese People’s Armed Police Forces, Shenyang 110034, China
Abstract： Objective To investigate the effect of gefitinib on the growth of human lung cancer cell line A549 and its molecular machenism. Methods The growth suppressing effect of gefitinib on A549 was quantified by MTT assay. Apoptosis was assayed with annexinⅤ-FITC/PI doubled stained flow cytometry. Western blotting assay was adopted to identify the expression of STAT3. Results After treatment with gefitinib, MTT showed that cell proliferation was inhibited, and the highest inhibitory rate was (80.20±0.45)% ( P ＜0.05). AnnexinⅤ-FITC/PI doubled stained flow cytometry showed that the cell apoptosis rate was (41.39±1.74)%, rising remarkably ( P ＜0.05). Western blotting showed significant decline of expression of STAT 3. Conclusions Gefitinib can significantly inhibit the growth of human lung cancer cell line A549 and induce early apoptosis. The effect may be due to the down-regulation of STAT 3 expression.
