Determination of three flavonoids in Apocynum venetum by high-performance capillary electrophoresis
LI Fang1,MA Xiaoli2,SUN Lian3,and MENG Lei2. 1. Department of Pharmacy,Xinjiang Production and Construction Corps Hospital of Chinese People’s Armed Police Forces,Urumqi 830063,China; 2. Analytical & Testing Center,3. College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China
Abstract：Objective To establish a high-performance capillary electrophoretic (HPCE) method for simultaneous determination of contents of rutin, isoquercitrin，and quercetin in Apocynum venetum. Methods A bare fused-silica capillary (58.5 cm×75 μm, effective length of 48.5 cm)was used. The buffer was composed of 50 mmol/L Na2B4O7 (pH 9.60). The running voltage was set at 20 kV with polarity from anode (inlet) to cathode (outlet). The separation time was 20 min and temperature was set at 25 ℃. Online detection was performed at 350 nm (PDA). Results The three types of flavonoids were well separated in 25 minutes and linear relationship in the peak area was good. The linear range was 4.4-44, 2.8-28.2, and 3.8-38 μg/ml, respectively. The correlation coefficient was 0.9968, 0.9995, and 0.9991, respectively. The average recovery was 97.3%, 98.4%, and 98.3%, respectively. Conclusions This method is simple, rapid, accurate, and reliable, which can be used for content determination of flavonoids in Apocynum leaves.
李 芳,马晓丽,孙 莲,孟 磊. 毛细管电泳法测定罗布麻叶中芦丁、异槲皮苷和槲皮素的含量[J]. 武警医学, 2012, 23(10): 859-861.
LI Fang,MA Xiaoli,SUN Lian,and MENG Lei. . Department of Pharmacy,Xinjiang Production and Construction Corps Hospital of Chinese People’s Armed Police Forces,Urumqi,China; . Analytical & Testing Center,. College of Pharmacy,Xinjiang Medical University,Urumqi,China. Determination of three flavonoids in Apocynum venetum by high-performance capillary electrophoresis. Med. J. Chin. Peop. Armed Poli. Forc., 2012, 23(10): 859-861.
2012, Vol. 23 
