Influence of venous leukocyte depletion on CD11/18 and CD14 leukocytes in canine Escherichia coli sepsis
CAI Zhiyang1,QIAN Baohua2,ZOU Yiqing3,WANG Liping4,and DENG Xiaoming5.
1. Department of Anesthesiology, Hospital of Corps 8710, Chinese People’s Armed Police Forces, Putian 351133, China; 2. Department of Blood Transfusion, 5. Department of Anesthesiology, Changhai Hospital, Shanghai 200433,China; 3. Department of Anesthesiology, No. 476 Hospital of Fuzhou Military Command General Hospital of PLA, Fuzhou 350102, China; 4. Department of Anesthesiology, Fuzhou Military Command General Hospital of PLA, Fuzhou 350025, China
Abstract： Objective To study the influence of venous leukocyte depletion on CD11/18 and CD14 leukocytes in canine Escherichia coli sepsis. Methods Canine models of Escherichia coli sepsis were established by infusing the live Escherichia coli (O111∶B4) via vein. Eighteen hybrid dogs were anesthetized intravenously and randomly divided into two groups: the filtration group and the control group. The filtration group dogs underwent venous leukocyte depletion. T0′was set at the time of beginning infusion of the live Escherichia coli (O111∶B4), T0 was set at the time of beginning venous leukocyte depletion. Mean arterial pressure, pulse, body temperature, red blood cell concentration, platelet concentration, leukocyte concentration, TNF-α concentration, IL-6 concentration in serum, CD11/18 expression, and CD14 expression in both groups were measured at T0′, T0, T1 and T5. Results There were no significant differences in pulse, red blood cell concentration, platelet concentration, TNF-α concentration, and IL-6 concentration between the two groups. Mean arterial pressure at T1 in the filtration group was greater than that in the control group (P<0.05). The body temperature and leukocyte concentration at T1 in the filtration group was greater than that in the control group (P<0.05). CD11/18 expression of neutrophils at T5 in the filtration group was lower than that in the control group (P<0.05). CD14 expression of monocytes at T1, T5 in the filtration group was lower than that in the control group (P<0.05). Conclusions Venous leukocyte depletion improves canine hemodynamics, depresses leukocyte concentration, depresses the expression of the CD11/18 of neutrophils, CD14 of monocytes, whereas does not affect the inflammatory factor in serum.
2013, Vol. 24 
