Abstract：Objective To study the dynamic changes in serum miRNA-122 levels during sepsis and the correlation between serum miRNA-122 and sepsis severity. Methods Fifty-two sepsis patients (including 23 non-severe septic patients and 29 severe septic patients)and 23 healthy controls were recruited in the study. The expression levels of serum miRNA-122 were quantified by quantitative reverse transcriptase polymerase chain reaction assays (qRT-PCR).Levels of serum miRNA-122 were detected on the 1 st, 3 rd, 5 th, 7 th, 10 th and 14 th day after their admissions in intensive care unit. All the patients clinical information was also recorded. Results After comparison, levels of serum miRNA-122 in sepsis patients on every observation time point were all significantly lower than those in healthy controls (P＜0.01). The receiver operating characteristic curve was obtained and the area under curve was calculated on the levels of serum miRNA-122 on the first day of their admissions. When the cut-off point was set at 0.710, miRNA-122 had a specificity of 95.7% and a sensitivity of 53.8%. The expression levels of serum miRNA-122 in severe sepsis group were significantly higher than in sepsis group on each observation time point (P＜0.01). Conclusions Serum miRNA-122 can used as a specific biomarker for diagnosis of sepsis. And miRNA-122 might be correlated with the organ injuries of septic patients.
Wang H, Zhang P, Chen W, et al. Evidence for serum miR-15a and miR-16 levels as biomarkers that distinguish sepsis from systemic inflammatory response syndrome in human subjects[J]. Clin Chem Lab Med, 2012, 50(8): 1423-1428.
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2013, Vol. 24 
