Effects of polysaccharides from Radix codonopsis on memory disorder of lead poisoning mice
ZHANG Li1,LI Dan1,LIU Jiping1,CHEN Yadan2,and MA Haiming3.
1. Jilin Provincial Corps Hospital of CAPF, Changchun 130052,China;
2. The Second Hospital of Jilin University, Changchun 130041, China;
3. Medical Department,The China-Japan Union Hospital of Jilin University, Changchun 130033,China
Abstract：Objective To study the effect of polysaccharides from Radix codonopsis ( PRC ) on memory disorder of lead poisoning mice and to explore its mechanism. Methods The learning ability of lead poisoning mice were detected by Morris water maze and step-down test, the activity of super oxide dismutases ( SOD ) in brain was determined by the nitrite formation and the content of malondialdehyde ( MDA ) in brain was determined by thiobarbituric acid colorimetric method,nitrobenzoic acid method was used to detect the content of acetylcholine esterase ( AchE ). Results During Morris water maze test ,compared with model group, in the middle and high dose of PRC groups mice, the latent period to reach the platform was significantly reduced (P<0.05) and the time to across the plat was significantly increased (P<0.05) . In step-down test, compared with model group , the learning training and memory test was significantly prolonged (P<0.01), the mistakes were significantly decreased (P<0.05), and electrifying time was significantly lowered (P<0.01). The activities of SOD were decreased and the content of MDA was increased in brain tissue of lead poisoning mice in the middle and high dose of PRC groups (P<0.05).There was no significant difference in AchE content. Conclusions PRC enhances the ability of learning and memory in lead poisoning mice. The mechanism may in improving lipid peroxidation and free radical scavenging.Further study of AchE content in brain in brain tissue of lead poisoning mice is needed.
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2013, Vol. 24 
