丙型肝炎病毒核酸定量和抗-HCV血清学分析的诊断价值

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摘要目的探讨PCR-荧光探针法与抗-HCV检测在丙型肝炎(丙肝)诊断中的应用价值。方法回顾性分析265例疑似丙肝病毒感染者行HCV-RNA、抗-HCV及ALT检测的资料,统计不同年龄、性别分布情况,进行HCV-RNA与抗-HCV、ALT的相关性分析。结果(1)男性抗-HCV与HCV-RNA的阳性率均高于女性,但差异无统计学意义(P>0.05);年龄>40岁的患者抗-HCV与HCV-RNA的阳性率明显高于年龄≤40岁的患者,差异有统计学意义(P<0.05)。(2)ALT异常率随HCV-RNA阳性率的增高而增加,并与HCV-RNA含量成正相关(r=0.999,P<0.05)。但ALT的水平与HCV-RNA含量无相关性(r=0.800,P>0.05)。(3)HCV-RNA阳性多伴有抗-HCV阳性(r=0.320),二者有很好的相关性。结论ELISA法检测抗-HCV在丙型肝炎诊断中仍旧为首选检测方法。联合应用HCV-RNA和抗-HCV及ALT检测,是诊断丙型肝炎最为可靠的方法。

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	刘贤华
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	荣冉
	刘维维

关键词 ：丙型肝炎病毒核酸定量检测, 抗-HCV, 诊断

Abstract：Objective To study the application value of hepatitis C virus RNA detection (PCR-fluorescent probe) and hepatitis C virus antibody detection (ELISA, anti-HCV) in the diagnosis of hepatitis C. Methods The HCV-RNA and anti-HCV tests of 265 suspected HCV patients were reviewed, statistics of anti-HCV and HCV-RNA detection results and ALT level in different ages and gender was collected and analysized. Results ①Anti-HCV and HCV-RNA positive rates in men were higher than those in women, but there was no statistically significant difference (P>0.05).② Anti-HCV and HCV-RNA positive rates in＞40 years old patients were obviously higher than those in ≤40 years old patients ,with statistically significant difference (P<0.05); ALT abnormal rates also had significant difference between＞40 and ≤40 years old patients (P<0.05).③ ALT abnormal rate increased with increasing HCV-RNA positive rate and have positive correlation with HCV-RNA content(r=0.999,P<0.05). But the ALT levels did not correlate with HCV-RNA content (r=0.800,P>0.05). ④The abnormal rate of Anti-HCV was closely correlated with HCV-RNA (r=0.320); Anti-HCV, HCV-RNA and ALT had no statistically significant difference (P>0.05). Conclusions ①Anti-HCV and HCV-RNA detection have no relation with gender, but they have certain relevance with age. ②ELISA for detecting anti-HCV is still first choice in HCV. Combined use of HCV-RNA,anti-HCV and ALT detection, will be the most reliable and valuable method in diagnosis of HCV.

Key words： HCV RNA quantification HCV antibody diagnosis

收稿日期: 2013-07-02

ZTFLH:

R512.63

通讯作者: 白晓东,E-mail;baixiaotmu@yahoo.com.cn E-mail: liuxianhuatmu@yahoo.com.cn

作者简介: 刘贤华,博士,主管技师,E-mail:liuxianhuatmu@yahoo.com.cn

引用本文:

刘贤华,白晓东,刘元明,段萃娟,荣冉,刘维维. 丙型肝炎病毒核酸定量和抗-HCV血清学分析的诊断价值[J]. 武警医学, 2013, 24(6): 488-490. LIU Xianhua,BAI Xiaodong,LIU Yuanming,DUAN Cuijuan,RONG Ran,and LIU Weiwei.. Retrospective analysis of Antibody of HCV and quantification of HCV RNA in diagnosis of hepatitis C. Med. J. Chin. Peop. Armed Poli. Forc., 2013, 24(6): 488-490.

链接本文:

http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2013/V24/I6/488

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