Experimental study on changes of serum total IgE, IL-4 and IL-5 in mice with Trichinella spiralis induced allergic asthma
MA Ping1,YAN Yuwen1,and BAO Chunyu2.
Department of Pathogenic biology and Immunology;
2. Academic Achievement office, Department of Scientific Research, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
Abstract：Objective To study the effect of Trichinella spiralis infection on total IgE, IL-4 and IL-5 in serum of the mice with allergic asthma. Methods Twenty female BALB/c mice were randomly divided into four groups of five mice each, including Ⅰ as control group, Ⅱ as allergic asthma group, Ⅲ as asthma followed by Trichinella spiralis infection, Ⅳ as Trichinella spiralis infected group. Firstly, the animals in group Ⅲ and Ⅳ were infected with 200-300 Trichinella spiralis muscle larvae. Twenty-eight days later, ovalbumin (OVA) was used to induce the allergic asthma for mice in group Ⅱ and Ⅲ, in order to establish asthma animal model. After 56 days, all mice were sacrificed, levels of TIgE, IL-4 and IL-5 in serum were measured by ELISA. Results The TIgE levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were (61.79±25.79) ng/ml,(437.08±75.68) ng/ml, (251.64±107.27) ng/ml and (446.12±74.32)ng/ml, respectively. The IL-4 levels in groupⅠ,Ⅱ, Ⅲ and Ⅳ were (136.49±31.17) pg/L, (209.80±21.57) pg/L, (162.79±17.69) pg/L and (182.71±12.08)pg/L, respectively. IL-5 levels in groupⅠ,Ⅱ, Ⅲ and Ⅳ were (7.31±1.86) ng/L, (16.00±2.29) ng/L,(9.24±1.73) ng/L and (11.41±2.11)ng/L, respectively. Compared with group Ⅱ, the TIgE、IL-4 and IL-5 levels in group Ⅲ decreased (P<0.05). Conclusions Trichinella spiralis infection can inhibit expression of the TIgE, IL-4 and IL-5 in serum of mice with allergic asthma
马萍,闫玉文,包春雨. 旋毛虫感染对过敏性哮喘小鼠血清总IgE、IL-4、IL-5的影响[J]. 武警医学, 2013, 24(6): 502-504.
MA Ping,YAN Yuwen,and BAO Chunyu. . Experimental study on changes of serum total IgE, IL-4 and IL-5 in mice with Trichinella spiralis induced allergic asthma. Med. J. Chin. Peop. Armed Poli. Forc., 2013, 24(6): 502-504.
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2013, Vol. 24 
