Effect of Xijiao Dihuang decoction on plasma cytokines in D-GalN/LPS induced mouse acute hepatic failure model
ZHANG Jinliang1,LIU Li2,GUO Ming1,LI Yongguang2,GUO Muohua1,and SUN Jun2
1.Department of traditional Chinese medicine,
2. Medical Department,
3.Department of General Medicine,Yunnan Provincial Corps Hospital, Chiese People’s Armed Police Forces, Kunming 650111, China
Abstract：Objective To study the effect of Xijiao Dihuang decoction on the plasma cytokine levels of D-galactosamine (D-GalN)/lipoplysaccharides (LPS) induced acute liver failure mouse model, and to elucidate the potential mechanism responsible for the protective effect on this mouse model by Xijiao Dihuang decoction. Methods The mouse model of acute liver failure was developed by intraperitoneally injecting D-GalN/LPS. The Xijiao Dihuang group and the control group were intragastically administered with Xijiao Dihuang decoction and double distilled water, respectively. The cytokine level 3 h after treatment in each group was assayed by cytometric bead assay. Results The level of Tumor necrosis factor-α (TNF-α) in the Xijiao Dihuang group was (709.44±336.12) pg/ml. Compared with the control group, no significant TNF-α reduction was observed in the mouse model of acute liver failure 3 h after Xijiao Dihuang decoction treatment. In contrast, the level of IL-10 in Xijiao dihuang group was (994.67±589.63)pg/ml 3 h after treatment that was significantly elevated as compared with the control group (P<0.05). Conclusions Xijiao Dihuang decoction generates protective effect on the mouse model of acute liver failure. The underlying mechanism may be up-regulation of IL-10 production rather than reduction of TNF-α secretion, thereby inhibiting the enhanced proinflammatory responses.
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2013, Vol. 24 
