Establishment of a Raw-264 cell model for evaluating virulence of Vibrio parahaemolyticus
MA Lizhi1,2,GUO Liping2,WANG Lixiang1,ZHAO Zhibing3,ZHANG Guangzhou3,ZHOU Dongsheng2,and QIU Yefeng3.
1.Department of Emergency Medicine, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039; 2.Beijing Institute of Microbiology and Epidemiology,State Key Laboratory of Pathogen and Biosecurity; 3.Laboratory Animal Center, Academy of Military Medical Sciences, Beijing 100071,China
Abstract：Objective To establish a Raw-264 cell model for evaluating virulence of Vibrio parahaemolyticus. Methods The CytoTox 96 Non-Radioactive Cytotoxicity Assay Kit was employed to test the Vibrio parahaemolyticus-induced cytotoxicity against Raw264 cells. Results The protocols were optimized as follows: Vibrio parahaemolyticus cells and Raw264 cells were incubated for 1.5 h at 37℃ with a MOI of 5; the mixture was then pelleted, and the 10-fold diluted supernatant was incubated with the substrate for 10 min. The cytotoxicity against Raw-264 cells determined for the opaR mutant was significantly higher than that for the wild-type strain. Conclusions A Raw-264 cellular model was established for evaluating virulence of Vibrio parahaemolyticus.
2013, Vol. 24 
