A study on analgesic effect of bufalin and its underlying mechanisms
YANG Guang,YANG Yu,ZHANG Yujie,and ZENG Xianyang
YANG Guang, YANG Yu, ZHANG Yujie, and ZENG Xianyang. Department of Anesthesiology , Liaoning Provincial Corps Hospital of Chinese People’s Armed Police Forces. Shenyang 110034, China
Abstract：Objective To investigate the analgesic effect of bufalin and its underlying mechanisms. Methods The analgesic effect of bufalin was studied by the acetic acid-induced writhing response and hot plate test. The contents of NO and the activation of MAPK/p38/iNOS pathway in LPS-induced RAW264.3 were measured by Griess assay and Western blotting, respectively. Results Bufalin (0.5, 1.0 mg/kg) showed a significant inhibition of acetic acid-induced writhing response and prolonging of licking time in hot plate test at a dose-dependent manner (P<0.05). Bufalin (0.04, 0.08 μM) significantly reduced the contents of NO and inhibited the activation of MAPK/p38/iNOS in RAW264.7 cells. Conclusions The analgesic of bufalin is related to suppressing the activation of MAPK/p38/iNOS pathway and subsequently reducing the synthesis and releases of excessive NO.
杨 光,杨 宇,张玉杰,曾宪阳. 蟾毒灵镇痛作用及其作用机制探讨[J]. , 2014, 25(3): 228-230.
YANG Guang,YANG Yu,ZHANG Yujie,and ZENG Xianyang. A study on analgesic effect of bufalin and its underlying mechanisms. , 2014, 25(3): 228-230.
2014, Vol. 25 
