1.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; 2.Department of Infection Gastroenterology,Children’s Hospital of Chongqing Medical University, Chongqing 400014, China
Abstract：Objective To investigate the prevalence, genotypes and epidemic characteristics of human bocavirus associated with acute pediatric diarrhea in Chongqing area. Methods Pediatric patients with acute diarrhea were recruited and stool samples were collected in Children’s Hospital of Chongqing Medical University during 2011. Real-time PCR and nested PCR were performed to determine the presence of HBoV and their genotypes. Results A total of 504 stool specimens were collected, from which 49 HBoV positive samples were identified, with a detection rate of 9.72%. A seasonal distribution of HBoV was observed, with a peak (19.72%) in summer. No distribution of age or gender specific was obtained for the HBoV positive detection. Based on Blast and MEGA analysis, 25 HBoV1, 23 HBoV2 and 1 HBoV3 were determined. All the HBoV2 strains detected belonged to HBoV-2B genotype. Conclusions The detection rate of HBoV has obvious seasonality, with a peak in summer. HBoV1 and HBoV-2B are the major genotypes.
Wang Y, Gonzalez R, Zhou H, et al. Detection of human bocavirus 3 in China [J]. Eur J Clin Microbiol Infect Dis, 2011, 30(6): 799-805.
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2014, Vol. 25 
