Abstract：Objective To study the expression level and correlation between CML28 and WT1 in acute leukemia(AL) patients’ bone marrow( BM) primary cells.Methods Bone marrow mononuclear cells (MMNC) were collected from 29 AL patients who were initially treated and admitted to the Tongji Hospital affiliated to Huazhong University of Science＆Technology from January to June in 2009.Total mRNA was extracted from MMNC of AL patients using Trizol reagents, and the first cDNA was synthesized using reverse transcription kits. Their mRNA expression levels were detected in AL patients by RT-PCR and real time PCR and their mRNA expression levels were quantitated by relevant quantitative formula 2-ΔΔCT.Then the correlation coefficient was calculated using SPSS 17.0 statistical software. Results There was a high expression level of CML28 and WT1 in the MMNC of AL patients at the same time, with significant difference between their mRNA expression leuels (r=0.462,P=0.008). Conclusions CML28 and WT1 are highly expressed in a variety of acute leukemia, and they have an obviously positive correlation.
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2014, Vol. 25 
