P63蛋白在肺癌中表达的定量分析及其与癌细胞凋亡的关系

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摘要目的对P63蛋白在正常肺脏及肺癌组织中的分布进行定量分析,判断其表达与细胞凋亡的关系。方法收集肺癌组织标本及肿物周围正常肺组织,制备组织芯片。以免疫组织化学方法鉴定P63表达并进行量化分析;以脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法判断细胞凋亡并进行量化分析;最终将结果量化为阳性单位(PU)进行分析。结果肺鳞癌细胞核P63的PU值大于正常肺支气管上皮、肺腺癌、大细胞癌、小细胞癌细胞核P63的PU值(P＜0.01);肺腺癌、大细胞癌、小细胞癌的PU值小于正常肺支气管上皮细胞核P63的PU值(P＜0.01);正常肺组织中P63的表达与细胞凋亡之间无相关关系(r＝0.1619,P＞0.05);肺癌组织中P63的表达与细胞凋亡呈正相关(r＝0.3333,P＜0.001)。结论正常肺组织中P63的表达量和细胞凋亡是两个独立存在的事件;而肺癌组织中P63的表达与细胞凋亡呈正相关。

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关键词 ： P63, 肺癌, 凋亡, 定量分析

Abstract：Objective To analyze the expression of protein p63 in normal lung and lung cancers quantitatively and judge its relationship with cellular apoptosis. Methods Lung cancer and normal lung tissues were collected and tissue arrays were prepared. The p63 expression was determined by immunohistochemistry and analyzed quantitatively. The cellular apoptosis was analyzed quantitatively by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). The final qualified results (positive unit, PU) were analyzed statistically. Results The PU values of the intensity of p63 protein expression in normal lung tissue, adenocarcinoma, large cell carcinoma and small cell carcinoma were all lower than that in squamous carcinoma (P＜0.01); The PU value of normal lung tissue was higher than those in adenocarcinoma, large cell carcinoma and small cell carcinoma (P＜0.01); while there was a positive correlation between P63 protein expression and apoptosis in all kinds of lung cancer (P＜0.01). Conclusions The extents of protein p63 expression are distinct on the normal lung and lung carcinoma tissues. The p63 expression and cellular apoptosis on the normal lung tissues are separated. However, p63 expression and cellular apoptosis on the lung malignant tissues are positively correlated.

Key words： p63 lung cancer apoptosis quantitative analysis

收稿日期: 2014-07-05

ZTFLH:

R734.2

通讯作者: 申洪,E-mail:shenhong2010168@163.com E-mail: luwang6@126.com

作者简介: 王璐,硕士,医师

引用本文:

王璐,申洪. P63蛋白在肺癌中表达的定量分析及其与癌细胞凋亡的关系[J]. 武警医学, 2014, 25(10): 1029-1031. WANG Lu SHEN Hong. Quantitative analysis of p63 expression in lung cancers and its relationship with cancer cellular apoptosis. Med. J. Chin. Peop. Armed Poli. Forc., 2014, 25(10): 1029-1031.

链接本文:

http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2014/V25/I10/1029

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