Construction of rat chronic stress depression model and its gastrointestinal mucosa change
ZHU Xiaohui1,2, ZHANG Chenggang1, LIU Haifeng1,2
1.Institute of Radiation Medicine, Academy of Military Medical Sciences; State Key Laboratory of Proteomics; Research Center for Military Cognition and Mental Hygiene of Chinese PLA, Beijing 100850, China; 2.Department of Gastroenterology, General Hospital of Armed Police Forces, Beijing 100039, China
Abstract：Objective To establish the rat chronic stress depression model and observe its gastrointestinal mucosa change rularity. To study the feasibility of establishing the chronic stress depression model based on chronic unpredictable stress method followed by behavioral evaluation, and further observe the histopathological changes in stomach, ileum, cecum and colon using HE staining. Methods The based method of chronic unpredictable stress was used. A total of 12 healthy male rats were randomly divided into two groups: control group and model group, six in each group. Rats in control group were regularly raised, and those in model group were randomly and alternately treated in 28 days with 10 stress methods, such as fasting for 24 h, water deprivation for 24 h, tail nipping (1 cm of rat tail head) for 5min, day and night inversion for 24 h, 4 ℃-cold water swimming for 5min, 45℃ environment for 5min, damp bedding for 24 h, sloping 45° for 24 h, behavior constraint for 4 h, horizontal vibrating (60 times/min) for 45 min. One method was chosen in each day and the interval of the same stimulation exceeded 7 days at least. Then, the rat body weight was recorded and the rats were on open field test, forced swimming test, and sugar water preference test. Finally, rats were dislocated and samples of stomach, ileum, cecum and colon were collected, and sliced and Haematoxylin and eosin staining was performed. Results Compared with the control group, the rat body weight significantly decreased (P=0.0064), the number of passing through significantly decreased (P=0.0001), the number of upright standing significantly decreased (P=0.0007), the number of grooming significantly decreased (P=0.0011), the no moving time in forced swimming significantly prolonged (P=0.000006) and sugar water preference significantly decreased(P=0.008152). Furthermore, the gastric mucosa and the microvilli in intestinal tract were seriously damaged, and nuclei slightly contracted and deeply stained. Conclusions Multiple chronic unpredictable stress method combination can correctly establish the rat chronic stress depression model, and the histology results demonstrate that the stress causes a signifcant damage in gastrointestinal mucosa.
朱晓慧, 张成岗, 刘海峰. 大鼠慢性应激抑郁模型的建立及其胃肠黏膜的变化[J]. 武警医学, 2015, 26(4): 336-339.
ZHU Xiaohui, ZHANG Chenggang, LIU Haifeng. Construction of rat chronic stress depression model and its gastrointestinal mucosa change. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(4): 336-339.
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2015, Vol. 26 
