Effects of acupuncture on hypoxia inducible factor -1α and local cerebral blood flow in rats with focal cerebral ischemia
ZHU Zaiqing1, XU Guigang1, GUO Yanli1, MA Bin1, LU Yan2
1.Department of Neurology, Shandong Provincial Corps Hospital, Chinese People’s Armed Police Forces,Jinan 250014,China; 2.Department of Experimental Acupuncturology,College of Acumox and Tuina,Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Abstract：Objective To observe the effect of acupuncture on hypoxia inducible factor -1α and local cerebral blood flow in rats with focal cerebral ischemia.Methods Wistar rats were randomly divided into four groups:normal group, sham-operated group, model group and electroacupuncture(EA) group. Focal cerebral ischemia rat models were developed by middle cerebral artery occlusion(MCAO )with thread embolus. Electroacupuncture was applied to bilateral PC 6 and LI11 after MCAO. The expression of hypoxia inducible factor -1α was examined in MCAO rats by Western blot and real-time quantitative PCR (qPCR).Results Acupuncture could promote the recovery of neurological function in MCAO rats. Western blot and real-time qPCR showed that acupuncture promoted the hypoxia inducible factor -1α expression in the ischemic brain tissue with statistical significance(P<0.05).Conclusion Acupuncture promotes hypoxia inducible factor -1α expression in rats with focal cerebral ischemia. All the results suggest that the increased expression of hypoxia inducible factor -1α in the ischemic brain tissue plays an important role in brain protection.
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2015, Vol. 26 
