Encapsulation efficiency of ozagrel hydroehloride liposome by HPLC
LIU Qing1,LIU Lifeng1, SUN Changyou1, Li Huifang2
1.Department of Pharmacy, Shanxi Provincial Corps Hospital, Chinese People’s Armed Police Forces, Taiyuan 030006, China; 2.Department of Pharmacy, Shanxi Labor Medical School,Taiyuan 030012,China
Abstract：Objective To establish a high performance liquid chromatography (HPLC) method for the encapsulation efficiency of ozagrel hydrochloride liposome. Methods The HPLC was used for determination. A C18 column was used with the mobile phase of 0.05 mol/L potassium dihydrogen phosphate solution (pH=7.0) - acetonitrile - methanol (80∶15∶5) at the detection wavelength of 274 nm. Results The calibration curve was linear in the concentration range of 10-100 μg/ml, r=0.9997. The inter- and intra- day RSD was less than 0.5 %. The method recoveries were (100.2±0.42) %. The average entrapment efficiency of tartrate indoquinoline liposome was (94.89±0.44)%. Conclusions This method is simple,accurate and can be used to determine the encapsulation efficiency of ozagrel hydroehloride in ozagrel hydrochloride liposome.
2015, Vol. 26 
