乙型肝炎前S1、S2抗原与HBV-DNA的相关性分析

摘要
图/表
参考文献
相关文章 (5)

全文: PDF (517 KB)
输出: BibTeX | EndNote (RIS)

摘要目的对比分析乙型肝炎(乙肝)前S1、S2抗原和HBV-DNA的相关性,探讨乙肝前S1、S2抗原的临床应用价值。方法随机选取乙肝患者420例,采用ELISA法对前S1、前S2抗原进行检测,采用荧光定量PCR对HBV-DNA进行检测,从血清学标志类型、HBV-DNA载量和HBV感染类型角度对HBV-DNA和前S1、S2抗原检测结果进行分析。结果乙肝大三阳患者乙肝前S1、S2抗原和HBV-DNA阳性率分别为98.48%、95.45%、100.0%;小三阳患者乙肝前S1、S2抗原和HBV-DNA阳性率分别为53.25% 、45.45% 、46.75%。HBsAg(+)HBcAb(+)患者乙肝前S1、S2抗原和HBV-DNA阳性率分别为53.75%、43.28%、44.78%。在高HBV-DNA载量组中乙肝前S1、S2抗原检出率较高。结论乙肝前S1、S2抗原与HBV-DNA有较好的相关性,可作为反映病毒感染与复制的指标。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	黄艳萍
	祝峰
	王新元
	邹皓琳
	许诺

关键词 ：前S1抗原, 前S2抗原, HBV-DNA

Abstract：Objective To study the relationship between HBV Pre-S1 antigen,HBV Pre-S2 antigen and HBV-DNA and investigate the clinical significance of the hepatitis B virus (HBV). Methods The markers Pre-S1 antigen, Pre-S2 antigen were determined by ELISA and HBV-DNA by the fluorescence quantitative PCR in 420 patients with HBV collected from September 2013 to May 2014. HBV, Pre-S1 antigen and Pre-S2 antigen results were analysed in different aspects, such as serum indices, HBV-DNA amounts and HBV-DNA infection types. Results In “big sanyang” patients the positive rates of Pre-S1, S2 antigen and HBV-DNA were 98.48%, 95.45%, 100%,respectively.In “small Sanyang” patients, the positive rates of Pre-S1, S2 antigen and HBV-DNA were 53.25%, 45.45%, 46.75%,respectively.In HBsAg+ HBcAb+ patients the positive rate of Pre-S1, S2 antigen and HBV-DNA were 53.75%, 43.28%, 44.78%,respectively.And the detection rates of HBV Pre-S1 antigen, HBV Pre-S2 antigen were higher in patients infected with high level of HBV-DNA. Conclusions There is a good correlation between HBV Pre-S1 antigen, HBV Pre-S2 antigen and HBV-DNA, which can reflect virus infection and replication.

Key words： Pre-S1Ag Pre-S2Ag HBV-DNA

收稿日期: 2014-09-23

ZTFLH:

R512.62

通讯作者: 祝峰,E-mail:zfwjjszd@163.com

作者简介: 黄艳萍,本科学历,主管技师,E-mail:765511283@qq.com

引用本文:

黄艳萍, 祝峰, 王新元, 邹皓琳, 许诺. 乙型肝炎前S1、S2抗原与HBV-DNA的相关性分析[J]. 武警医学, 2015, 26(6): 600-602. HUANG Yanping, ZHU Feng, WANG Xinyuan,ZOU Haolin, XU Nuo. Relationship between hepatitis B virus former S1 antigen,former S2 antigen, and HBV-DNA, and their clinical significance. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(6): 600-602.

链接本文:

http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2015/V26/I6/600

[1]	丁天鹏,施根林,孙庆明,等.肝病患者血清胱抑素C水平变化及其临床意义[J].武警医学, 2013,24(4):302-305.
[1]	丁天鹏,施根林,孙庆明,等.肝病患者血清胱抑素C水平变化及其临床意义[J].武警医学, 2013,24(4):302-305.
[2]	杨绍基,任红. 传染病学[M].7版,北京:人民卫生出版社,2009:33-38.
[2]	杨绍基,任红. 传染病学[M].7版,北京:人民卫生出版社,2009:33-38.
[3]	Perz J F, Armstrong G L, Farrington, et al. The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide [J]. Hepatol, 2006, 45(4):529-538.
[3]	Perz J F, Armstrong G L, Farrington, et al. The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide [J]. Hepatol, 2006, 45(4):529-538.
[4]	Hsu J L, Chuang W J, Su I J, et al. Zinc-Dependent Interaction between JAB1 and Pre-S2 Mutant Large Surface Antigen of Hepatitis B Virus and Its Implications for Viral Hepatocarcinogenesis [J]. Virol, 2013, 87(23): 12675-12684.
[4]	Hsu J L, Chuang W J, Su I J, et al. Zinc-Dependent Interaction between JAB1 and Pre-S2 Mutant Large Surface Antigen of Hepatitis B Virus and Its Implications for Viral Hepatocarcinogenesis [J]. Virol, 2013, 87(23): 12675-12684.
[5]	Lok A S, Mcahon B J. Chronic hepatitis B [J] . Hepatology,2007,45(2):507-539.
[5]	Lok A S, Mcahon B J. Chronic hepatitis B [J] . Hepatology,2007,45(2):507-539.
[6]	Makvandi M, Neisi N, Khalafkhany D, et al. Occult Hepatitis B Virus Among the Patients With Abnormal Alanine Transaminase [J]. Jundishapur J Microbiol, 2014,7(8): 11648.
[6]	Makvandi M, Neisi N, Khalafkhany D, et al. Occult Hepatitis B Virus Among the Patients With Abnormal Alanine Transaminase [J]. Jundishapur J Microbiol, 2014,7(8): 11648.
[7]	邓燕玲.乙肝前s1抗原与乙肝五项阳性相关性的研究[J]. 安徽医药, 2013,17(6):1009-1010.
[7]	邓燕玲.乙肝前s1抗原与乙肝五项阳性相关性的研究[J]. 安徽医药, 2013,17(6):1009-1010.
[8]	顾春瑜,许琼,赵乔妹.化学发光技术检测前s1蛋白的临床价值[J]. 感染炎症修复, 2013,14(2):107-109.
[9]	张和平,林国跃,王萍,等. HBV前s2蛋白检测与HBV-DNA和乙肝二对半模式相关性研究[J]. 医学综述, 2011,17(1):139-140.
[8]	顾春瑜,许琼,赵乔妹.化学发光技术检测前s1蛋白的临床价值[J]. 感染炎症修复, 2013,14(2):107-109.
[9]	张和平,林国跃,王萍,等. HBV前s2蛋白检测与HBV-DNA和乙肝二对半模式相关性研究[J]. 医学综述, 2011,17(1):139-140.
[10]	Tong S P, Kim K H,Chante C, et al. Hepatitis B virus e antigen variants[J] . Inter J Med Sci, 2005, 2(1):2-7.
[11]	乐爱平,万腊根,郭萌萌,等.乙型肝炎病毒前s2抗原抗体系统检测的临床意义[J].江西医学检验,2003,21(5):323-326.
[12]	Kim B K, Choi S H, Ahn S H, et al. Pre-S mutations of hepatitis B virus affect genome replication and expression of surface antigens[J]. Gastroenterol Hepatol, 2014,29(4):843-850.
[10]	Tong S P, Kim K H,Chante C, et al. Hepatitis B virus e antigen variants[J] . Inter J Med Sci, 2005, 2(1):2-7.
[11]	乐爱平,万腊根,郭萌萌,等.乙型肝炎病毒前s2抗原抗体系统检测的临床意义[J].江西医学检验,2003,21(5):323-326.
[12]	Kim B K, Choi S H, Ahn S H, et al. Pre-S mutations of hepatitis B virus affect genome replication and expression of surface antigens[J]. Gastroenterol Hepatol, 2014,29(4):843-850.