Effection of EGB 761 to apoptosis of Ewing’s sarcoma VH-64 cell in vitro
LI Zhanning1, MENG Hongtao1, YAN Kun1,CUI Haibin2,GUO Yi2, LIU Jing1
1.Medical Department, Shanxi Provincial Corps Hospital of Chinese People’s Armed Police Forces, Xi’an 710054,China; 2. Hygiene Division of Logistics Department, Shanxi Provincial Corps of Chinese People’s Armed Police Forces, Xi’an 710054,China
Abstract：Objective To explore the contribution of EGB761 to the proliferation and apoptosis of Ewing’s sarcoma VH-64 cell in vitro. Methods CCK-8 assay was used to measure 50,100 and 200 mg/L which its effects on the proliferation of Ewing’s sarcoma VH-64 cell cultured with different concentrations of allicin for 12 h, 24 h, 48 h, AnnexinV-FITC and PI assay by flow cytometer (FCM) were applied to detect the apoptosis induced by EGB761. The changes of cell cycle were detected by FCM. Results EGB761 suppressed the proliferation of Ewing’s sarcoma VH-64 cell in a concentration-and time-dependent manner EGB761 induced cellular apoptosis of the Ewing’s sarcoma VH-64 cell in a concentration-dependent manner. With EGB761 100, 200 mg/L intervention Ewing’s sarcoma VH-64 cell, flow cytometer (FCM) test showed that the cell apoptosis rate were 6.85%±0.36% and 14.36%±0.23%,which were significant higher than that of control group (P< 0.01). Conclusions EGB761 significantly suppresses the growth of Ewing’s sarcoma VH-64 cell, induces the apoptosis of Ewing’s sarcoma VH-64 cell. Therefore resveratrol may be considered as a possible treatment medicine for Ewing’s sarcoma.
Park S G, Jung S, Ryu H H, et al. Role of 14-3-3-beta in the migration and invasion in human malignant glioma cell line U87MG[J]. Neurol Res, 2012, 34(9): 893-900.
[5]
Senthilkumar K, Arunkumar R, Elumalai P, et al. Quercetin inhibits invasion, migration and signalling molecules involved in cell survival and proliferation of prostate cancer cell line (PC-3)[J]. Cell Biochem Funct,2011, 29(2): 87-95.
Pradhan S J, Mishra R, Sharma P, et al. Quercetin and sulforaphane in combination suppress the progression of melanoma through the down-regulation of matrix metalloproteinase-9[J]. Exp Ther Med, 2010, 1(6): 915-920.
[3]
Bi J, Guo A L, Lai Y R, et al. Overexpression of clusterin correlates with tumor progression,metastasis in gastric cancer:a study on tissue microarrays[J]. Neoplasma,2010, 57(3): 191-197.
[7]
Lamoureux F, Thomas C, Yin M J, et al. Clusterin inhibition using OGX-011 synergistically enhances Hsp90 inhibitor activity by suppressing the heat shock response in castrate resistant prostate cancer[J]. Cancer Res, 2011, 71(17): 5838-5849.
[4]
Park S G, Jung S, Ryu H H, et al. Role of 14-3-3-beta in the migration and invasion in human malignant glioma cell line U87MG[J]. Neurol Res, 2012, 34(9): 893-900.
[8]
Watari H, Kinoshita R, Han Y, et al. Prognostic significance of clusterin expression in advanced stage cervical cancer treated with curative intended radiotherapy[J]. Int J Gynecol Cancer,2012,22(3):465-470.
[5]
Senthilkumar K, Arunkumar R, Elumalai P, et al. Quercetin inhibits invasion, migration and signalling molecules involved in cell survival and proliferation of prostate cancer cell line (PC-3)[J]. Cell Biochem Funct,2011, 29(2): 87-95.
[9]
Watari H, Kanuma T, Ohta Y, et al. Clusterin expression inversely correlates with chemosensitivity and predicts poor survival in patients with locally advanced cervical cancer treated with cisplatin-based neoadjuvant chemotherapy and radical hysterectomy[J]. Pathol Oncol Res,2010,16(3): 345-352.
[10]
Redondo M, Rodrigo I, Alcaide J, et al. Clusterin expression is associated with decreased disease-free survival of patients with colorectal carcinomas[J]. Histopathology,2010, 56(7): 932-936.
[6]
Pradhan S J, Mishra R, Sharma P, et al. Quercetin and sulforaphane in combination suppress the progression of melanoma through the down-regulation of matrix metalloproteinase-9[J]. Exp Ther Med, 2010, 1(6): 915-920.
[11]
Girard F P, Byrne J, Downes M, et al. Detecting solube clusterin in vitro and in vivo models of prostate cancer[J]. Neoplasma,2010, 57 (5): 488-493.
[7]
Lamoureux F, Thomas C, Yin M J, et al. Clusterin inhibition using OGX-011 synergistically enhances Hsp90 inhibitor activity by suppressing the heat shock response in castrate resistant prostate cancer[J]. Cancer Res, 2011, 71(17): 5838-5849.
[8]
Watari H, Kinoshita R, Han Y, et al. Prognostic significance of clusterin expression in advanced stage cervical cancer treated with curative intended radiotherapy[J]. Int J Gynecol Cancer,2012,22(3):465-470.
[9]
Watari H, Kanuma T, Ohta Y, et al. Clusterin expression inversely correlates with chemosensitivity and predicts poor survival in patients with locally advanced cervical cancer treated with cisplatin-based neoadjuvant chemotherapy and radical hysterectomy[J]. Pathol Oncol Res,2010,16(3): 345-352.
[10]
Redondo M, Rodrigo I, Alcaide J, et al. Clusterin expression is associated with decreased disease-free survival of patients with colorectal carcinomas[J]. Histopathology,2010, 56(7): 932-936.
[11]
Girard F P, Byrne J, Downes M, et al. Detecting solube clusterin in vitro and in vivo models of prostate cancer[J]. Neoplasma,2010, 57 (5): 488-493.
2015, Vol. 26 
