Significance of tissue cells in diagnosis of inflammatory bowel disease
XU Xin1,JI Xiaolong1, XU Mingzhi2
1. Department of Pathology,General Hospital of Chinese People’s Armed Police Forces,Beijing 100039,China; 2.the Second Clinic, National Defense University of PLA, Beijing 100063,China
Abstract：Objective Inflammatory bowel disease(IBD) includes ulcerative colitis(UC) and Crohn’s disease(CD). Definitive diagnosis of UC and CD relies on colonoscopy and biopsy, but it is difficult to give a definitive diagnosis for pathologists by specimens taken from colonoscopy. We hope that through the study for distribution and types of tissue cells within lesions ,looking for new clues to differential diagnosis.Methods Combining clinical history, endoscopy and the new standard, we remade the diagnosis, finally made sure of 30 cases of UC, 26 cases of CD, and 10 cases of normal intestine mucosa taken as the control. All the cases were re-stained by HE and two-step IHC for 6 items of antigens(CD1a、CD21、CD68、CD163、S-100、Ki-67), and these sections were analyzed by UNISCAN system to do semi-quota standard. The SPSS13.0 was used for statistics. The IHC was also analyzed through dividing the strength of the positive staining. Results The cell number in the CD and UC was significantly different from the control(P<0.01). In the CD mucosa, there were more positive cells than in the normal control and UC, with statistically significant difference(P<0.05).The CD21 expression could be the result of other cells stained, and had little diagnostic value. The S-100 positive cell types were rich in these 3 groups, in every two group with statistically significant difference(P<0.05).There was no CD68 expression statistically significant difference in the UC group compared with normal control(P>0.05), but the positive cell number in the CD group were less compared with the normal control and UC group, with statistically significant difference(P<0.05). There were rare Ki67 positive cells in the control group. In the UC group the Ki67 positive cells were more than in the CD group. Three groups of comparisons between groups were statistically significantly different (P<0.05).Conclusions These antibodies can be used as staining differential diagnosis of UC and CD. CD1a:UC(-),CD(+); Ki67 UC(+>10%),CD(+1%-2%);number of positive cells of S-100 and CD68, UC〉CD. The other antibodies can be used as auxiliary diagnositic means.
Caballero T, Nogueras F, Medina M T, et al. Intraepithelial and lamina proprialeuecxtyle subsets in inflammatory bowel disease: an immunohistocheical study of colon and biospy specimens[J]. J Clin Pathol, 1995, 48:743-748.
Steinman R M,Hawiger D,Liu K,et al. Dendritic cell funtion in vivo during the steady state: a role in peripheral tolerance [J]. Ann NY Acad Sci, 2003,987:15-25.
[5]
Caballero T, Nogueras F, Medina M T, et al. Intraepithelial and lamina proprialeuecxtyle subsets in inflammatory bowel disease: an immunohistocheical study of colon and biospy specimens[J]. J Clin Pathol, 1995, 48:743-748.
Steinman R M,Hawiger D,Liu K,et al. Dendritic cell funtion in vivo during the steady state: a role in peripheral tolerance [J]. Ann NY Acad Sci, 2003,987:15-25.
2015, Vol. 26 
