Effects of simulated weightlessness on DRG in female rats
REN Ningtao1, ZHANG Heng1, LI Jie2, LEI Wei4, ZHANG Ran3, ZHANG Yonggang1, CUI Geng1
1.Orthopedics Department, 2.Gynecology Department, 3.Cardiology Department, General Hospital of PLA, Beijing,100853,China; 4. Orthopedics Department, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
Abstract：Objective To observe the effect of simulated weightlessness on DRG in female rat model. Method 80 female Sprague-Dawley rats were randomly designated as HU groups (n=40) and normal control(NC) groups (n=40). Four weeks later, the L5 DRG was excised, lumbar 5 DRG were stained with HE staining, the myelin of DRG was observed by using immunohistochemistry. TEM method aimed to observe the DRG myelin and mitochondria. Results HE staining showed that, in HU groups, the arrangement between cells in DRG were slightly loose, some ganglion cells and satellite cells were separated. IOD in NC groups was 0.573±0.007, which was lower than in HU groups (0.802±0.009)(P<0.05). In HU group, the structure of myelin was disorderly and twisted. Mitochondria in HU group were swollen and deformed obviously. Conclusions Stimulated weightlessness can cause the damage changes in the DRG myelin and mitochondria. These suggests that simulated weightlessness can cause damage in female rat DRG.
Dai Z Q, Wang R, Ling S K, et al. Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells [J]. Cell Prolif, 2007,40:671-684.
[1]
Blaber E,Marcal H,Burns B P. Bioastronautics: the influence of microgravity on astronaut health [J]. Astrobiology, 2010,10(5):463 -474.
Ren X L, Zhang R, Zhang Y Y, et al. Nitric oxide synthase activity in the abdominal aorta of rats is decreased after 4 weeks of simulated microgravity [J]. Clin Exp Pharmacol Physiol, 2011, 38(10):683-687.
[6]
Kawano F, Nomura T, Ishihara A, et al. Afferent input-associated reduction of muscle activity in microgravity environment [J]. Neuroscience, 2002,114(4):1133-1138.
[3]
Jojo V S, Jeffrey L, Scott P,et al. Back pain in space and post-flight spine injury: mechanisms and counter measure development [J]. Acta Astronautica,2013,86:24-38.
[7]
Zhang R, Ran H H, Cai L L, et al. Simulated microgravity-induced mitochondrial dysfunction in rat cerebral arteries [J]. FASEB J, 2014,(6):1134-1135.
[4]
Dai Z Q, Wang R, Ling S K, et al. Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells [J]. Cell Prolif, 2007,40:671-684.
[8]
Williams D, Kuipers A, Mukai C, et al. Acclimation during space flight: effects on human physiology [J]. CMAJ, 2009, 180(13), 1317-1323.
Miranda-Saksena M, Boadle R A, Armati P, et al. In rat dorsal root ganglion neurons, herpes simplex virus type 1 tegument forms in the cytoplasm of the cell body [J]. J Virol, 2002, 76(19):9934-9951.
[6]
Kawano F, Nomura T, Ishihara A, et al. Afferent input-associated reduction of muscle activity in microgravity environment [J]. Neuroscience, 2002,114(4):1133-1138.
[10]
Ishihara A, Yamashiro J, Matsumoto A, et al. Comparison of cell body size and oxidative enzyme activity in motoneurons between the cervical and lumbar segments in the rat spinal cord after spaceflight and recovery[J]. Neurochem Res,2006,31(3):411-415.
[7]
Zhang R, Ran H H, Cai L L, et al. Simulated microgravity-induced mitochondrial dysfunction in rat cerebral arteries [J]. FASEB J, 2014,(6):1134-1135.
[8]
Williams D, Kuipers A, Mukai C, et al. Acclimation during space flight: effects on human physiology [J]. CMAJ, 2009, 180(13), 1317-1323.
[11]
Morey-Holton E R, Globus R K.Hindlimb unloading rodent model: technical aspects [J].J Appl Physiol, 2002,92(4):1367-1377.
[12]
Ren J C, Fan X L, Song X A, et al. Prolonged hindlimb unloading leads to changes in electrophysiological properties of L5 dorsal root ganglion neurons in rats after 14 days [J]. Muscle Nerve,2012,45(1):65-69.
[9]
Miranda-Saksena M, Boadle R A, Armati P, et al. In rat dorsal root ganglion neurons, herpes simplex virus type 1 tegument forms in the cytoplasm of the cell body [J]. J Virol, 2002, 76(19):9934-9951.
[10]
Ishihara A, Yamashiro J, Matsumoto A, et al. Comparison of cell body size and oxidative enzyme activity in motoneurons between the cervical and lumbar segments in the rat spinal cord after spaceflight and recovery[J]. Neurochem Res,2006,31(3):411-415.
[11]
Morey-Holton E R, Globus R K.Hindlimb unloading rodent model: technical aspects [J].J Appl Physiol, 2002,92(4):1367-1377.
[12]
Ren J C, Fan X L, Song X A, et al. Prolonged hindlimb unloading leads to changes in electrophysiological properties of L5 dorsal root ganglion neurons in rats after 14 days [J]. Muscle Nerve,2012,45(1):65-69.
2015, Vol. 26 
