Influence of urokinase on nerve cell apoptosis after cardiopulmonary resuscitation in rabbits
GUO Xiaodong1, ZHANG Wei2, GUO Jing3, SU Qingming4, YANG Guirong1,ZHANG Jie1,SUN Kun5,and WANG Lixiang5
1.The First Department of Critical Care Medicine, 3. Department of Medical Care, 4. Department of Pathology , 5. Department of Emergency,General Hospital of Chinese People's Armed Police Force, Beijing 100039,China; 2. Out-patient Department, Department of Information, General Staff Headquarters, Beijing 100840, China
Abstract：Objective To investigate the effect of urokinase on nerve cell apoptosis after cardiac arrest(CA) and cardiopulmonary resuscitation(CPR)in rabbits. Methods 20 rabbits were randomly divided into experimental group and control group, 10 rabbits in each group. Potassium chloride injection combined with asphyxia method was conducted to establish the CA models. CPR and basic life-support were performed in experimental group. Based on above treatments, intervention with urokinase (20 000 U/kg) was conducted in experimental group. Neural apoptosis was identified by TUNEL and caspase-3 was detected by immunohistochemistry in order to prove the effect of urokinase on nerve cell apoptosis in rabbits 24 hours after CPR. Results Tunel and caspase-3 positive cells counts of cerebral cortex and hippocampus in experimental group were significantly less than those in control group, respectively. The comparisons were as follows(Tunel positive cells in cerebral cortex 29.22±7.86: 37.20±7.04(t=-2.392,P=0.028), in hippocampus 18.80±7.58: 27.4±8.15(t=-2.442,P=0.025), Caspase-3 positive cells in cerebral cortex 22.1±9.93:33.1±12.64(t=-2.165,P=0.044),in hippocampus 31.3±15.23 vs 46.8±16.73(t=-2.167,P=0.044); so thrombolytic therapy with urokinase can relieve nerve cell apoptosis obviously. Comparison of spontaneous circulation restoration time(s) 307.9±96.4: 242.0±71.0(t=-1.741, P=0.099), spontaneous breathing restoration time(min) 20.6±12.5:19.3±10.0 (t=-0.256, P=0.801), mean arterial pressure MAP (mmHg) 65.5±6.2: 69.0±6.7 (t=1.217, P=0.239), there were no differences between the experimental group and control group. Conclusions Thrombolytic therapy with urokinase in CPR after CA can improve and lessen nervous lesion resulting from apoptosis in rabbits.
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2015, Vol. 26 
