miRNA-214 was involved in cardioprotection by electroacupuncture pretreatment of myocardial ischemia/ reperfusion injury in rats
LIU Peiyu1, TIAN Yi2, BAO Manxi3,WANG Yue1, and CUI Fen1
1. Department of Anesthiology, 3. Hospital Office, Hainan Municapal Corps Hospital, Chinese People’s Armed Police Forces, Haikou 570203, China; 2. Department of Anaesthesiology,Haikou Hospital,Xiangya Medical College,Central South Univercity,Haikou 570208,China
Abstract：Objective To evaluate whether miRNA-214 mechanism was involved in the cardioprotection by electroacupuncture pretreatment of myocardial ischemia/ reperfusion injury in rats. Methods 24 male SD rats were divided into 4 groups randomly (n=6): control group (sham group), sham + electric acupuncture group (Sham+EA) group, ischemia/reperfusion group (I/R group), ischemia/reperfusion + electric acupuncture group (I/R + EA group). Rats in group sham+EA and I/R+EA received electroacupuncture stimulation 30 min per day for 3 days before myocardial ischemia/reperfusion injury. Rats in group I/R and I/R+EA received left anterior descending (LAD) occlusion for 40min and reperfusion for 180 min. The indices of the cardiac function, myocardial infarct size , LDH and CK activities as well as expression of miRNA-214 were measured. Results All the indices of the cardiac function were not significantly different between the sham and the sham+EA group. Compared with the sham group, all the indices of the cardiac function decreased significantly in I/R group (P<0.05). I/R+EA group showed that all the indices of the cardiac function increased, compared with I/R group. Myocardial infarct size in I/R+EA group significantly decreased, compared with I/R group. The activity of LDH and CK in of I/R group significantly increased, compared with I/R+EA group (P<0.05 or P<0.01). The expression of miRNA-214 in sham, sham+EA, I/R and I/R+EA group were 1, 1.6, 1.75 and 2.82, separately. The expression of miRNA-214 in the I/R group was higher than in sham group. Electroacupuncture pretreatment increased miR - 214 expression in sham + EA group and the I/R + EA group. Conclusions Electroacupuncture pretreatment can protect the heart against myocardial ischemia/ reperfusion injury and can promote the expression of miRNA-214 in rats.
柳培雨，田毅，鲍缦夕，王玥，崔芬. 大鼠心肌缺血-再灌注损伤miRNA-214介导电针预处理的保护作用[J]. 武警医学, 2016, 27(1): 43-47.
LIU Peiyu, TIAN Yi, BAO Manxi,WANG Yue, and CUI Fen. miRNA-214 was involved in cardioprotection by electroacupuncture pretreatment of myocardial ischemia/ reperfusion injury in rats. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(1): 43-47.
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2016, Vol. 27 
