Effect of all-trans retinoic acid on the expression of GSK3B and gastric cancer stem cells related gene CD133, Sox2
LIU Dandan1,2,XIE Limin2,FENG Yanjing2,LI Jinyi1,and WANG Xiangdang3
1.Clinical Teaching and Research Section, 3. Purchasing Center,the General Hospital of Chinese People’s Armed Police Force, Beijing 100039, China; 2. Graduate School of Jinzhou Medical University, Jinzhou 121000, China
Abstract：Objective To investigate the effect of all-trans retinoic acid (ATRA) on related genes CD133,Sox2 and GSK3B of gastric cancer stem cells in order to find a new starting point for the treatment of gastric cancer.Methods Human gastric cancer xenografts in nude mice were divided into control group, 5-FU group, ATRA group and 5-FU+ATRA group. After intraperitoneal injection of ATRA, the general condition of nude mice were observed before the tumor-bearing mice were sacrificed to remove the tumor tissue. HE staining was used to detect gastric cancer cell necrosis of the tissue. The expression of CD133mRNA, Sox2mRNA and GSK3BmRNA was detected using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative PCR analysis while immunohistochemistry was adopted to detect the expression of CD133, Sox2 protein and GSK3B.Results Compared with the control group, the tumor shrank in 5-Fu group, ATRA group, and 5-Fu+ATRA group. The difference was significant (P<0.05). The expressions of CD133, Sox2 stem cell-related factors were high in 5 -Fu group, ATRA group, and 5-Fu+ATRA group. However, there was significant difference in GSK3B expression between ATRA group and 5-Fu+ATRA group, (P<0.05).Conclusions After the administration of ATRA, gastric cancer stem cell markers, such as CD133 and Sox2, have a higher expression. The increased expression of CD133, Sox2 and GSK3B might inhibit the growth of tumor cells. The new use of ATRA can contribute to the prognosis of gastric cancer.
刘丹丹,谢丽敏,冯延静,李锦毅,王向党. 全反式维A酸对胃癌干细胞相关基因CD133、Sox2及GSK3B表达的影响[J]. 武警医学, 2017, 28(2): 135-138.
LIU Dandan,XIE Limin,FENG Yanjing,LI Jinyi,and WANG Xiangdang. Effect of all-trans retinoic acid on the expression of GSK3B and gastric cancer stem cells related gene CD133, Sox2. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(2): 135-138.
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2017, Vol. 28 
