Abstract：Objective To investigate the damage of benzo(a)pyrene (BaP) to human placental trophoblast cells in vitro by observing the effect of BaP on the proliferation and apoptosis of these cells, and on expressions of aryl hydrocarbon receptor (AhR) and aryl hrdrocarbon receptor nuclear translocator (ARNT). Methods BaP concentrations were divided into four groups: blank control group, low dose group, medium dose group and high dose group.The JEG-3 cells were treated with different concentrations of BaP for 24 h. The MTT assay was used to measure cell growth, and flow cytometry was used to measure cell apoptosis rates. AhR and ARNT mRNA were detected by real-time qPCR, while AhR and ARNT proteins were detected by Western blot. Results The cell proliferation rates in the medium dose group and high dose group were lower than those of the blank control group and low dose group (P<0.05). The apoptotic rate of the medium dose group and high dose group was higher than that of the blank control group and low dose group (P<0.05). The expressions of AhR gene(1.71±0.15、2.10±0.17和2.29±0.16) and ARNT gene(1.57±0.13、1.96±0.17和2.36±0.15)in low, medium and high dose groups of BaP were higher than that in the blank control group(1.41±0.13、1.28±0.11)(P<0.05), which changed in a concentration-dependent manner.The expressions of AhR protein(29.54±2.34、34.64±2.35 and 39.96±2.36) and ARNT protein(39.94±2.37、45.62±2.41 and 51.52±2.66)in low, medium and high dose groups of BaP were higher than those in the control group(23.22±2.31、34.28±2.24、39)(P＜0.05),which also changed in a concentration-dependent manner. Conclusions BaP can inhibit the cellular proliferation and induce apoptosis in human placental trophoblast cells in vitro, and the AhR pathway activation may be one of the mechanisms by which BaP damages human placental trophoblast cells.
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2019, Vol. 30 
