p53调控信号转导子和转录激活子3对肺癌细胞增殖及凋亡的影响

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摘要目的探讨p53调控信号转导子和转录激活子3(STAT3)对肺癌细胞增殖及凋亡的影响。方法细胞分组为空白对照组、阴性对照组、p53 小干扰RNA(siRNA)组、p53 siRNA+STAT3 siRNA组,采用western blot法检测组织中p53和STAT3蛋白表达水平;采用Transwell法检测转染后体外迁移能力和侵袭能力;采用流式细胞术膜联蛋白Ⅴ/碘化丙啶(AV-PI)双染法检测转染后凋亡情况。结果肺癌组织中p53表达水平明显下调,而STAT3表达阳性率增高,差异均有统计学意义(P<0.05)。转染p53的siRNA能显著上调肺癌细胞中STAT3的表达水平;p53 siRNA组和p53 siRNA+STAT3 siRNA组A549细胞凋亡率(17.91%±0.67%,22.51%±0.56%)、细胞的迁移速度[(55.16±6.50)μm/h,(63.30±5.98)μm/h]、穿膜细胞数(58.23±7.12,68.23±7.14),与空白对照组和阴性对照组比较,差异均有统计学意义(P<0.05)。结论 p53在肺癌中呈现低表达,p53能负向调控STAT3的表达,从而抑制其增殖和凋亡能力。

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	赵振山
	李海洋
	赵振兴
	代岱
	郝孟辉

关键词 ：肺癌, p53, 信号转导子和转录激活子3, 细胞生长, 细胞迁移, 细胞凋亡

Abstract：Objective To investigate the expression of p53 in lung cancer and its effect on proliferation and apoptosis of lung cancer cells by regulating signal transducers and activators of transcription 3 (STAT3) signaling pathway.Methods Cells were divided into blank control group, negative control group, p53 small interference RNA (siRNA) group and p53 siRNA+STAT3 siRNA group. The expressions of p53 and STAT3 were detected by Western blotting. Transwell assay was used to measure cell migration and invasion, while flow cytometry using Annexin V/propidium iodide (AV-PI) double staining was adopted to detect cell apoptosis.Results The level of p53 expression in lung cancer tissues was significantly lower, while the positive rate of STAT3 expression was increased (all P<0.001). SiRNA transfected with p53 could significantly increase the expression level of STAT3 in lung cancer cells. The growth and migration ability of cells in p53 siRNA group was significantly increased, while the level of apoptosis was significantly reduced (P<0.001).Conclusion P53 shows low expressions in lung cancer, and can negatively regulate the expression of STAT3, thus inhibiting the ability of proliferation and apoptosis.

Key words： lung cancer p53 signal transducer and activator of transcription 3 cell growth cell migration cell apoptosis

收稿日期: 2018-12-10

ZTFLH:

R734.2

作者简介: 赵振山,硕士,主治医师。

引用本文:

赵振山, 李海洋, 赵振兴, 代岱, 郝孟辉. p53调控信号转导子和转录激活子3对肺癌细胞增殖及凋亡的影响[J]. 武警医学, 2019, 30(5): 402-405. ZHAO Zhenshan, LI Haiyang, ZHAO Zhenxing, DAI Dai, HAO Menghui. Downregulation of p53 promotes cell proliferation and migration and inhibits apoptosis by targeting signal transducers and activators of transcription 3 in lung cancer. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(5): 402-405.

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http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2019/V30/I5/402

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