Expression of Hec-1 in bladder cancer and its clinical significance
SU Hong1, SONG Bo2, ZHANG Shanshan1, LI Qing3, YU Xinlu1, WANG Xue4, GAO Wanfeng1
1.Department of Surgery,2. Department of Pharmacy, 3. Department of urology Medical Office,4. Department of Rehabilitation division,Liaoning Provincial Corps Hospital,Chinese People’s Armed Police Force, Shenyang 110034, China
Abstract：Objective To investigate the expression of highly expressed protien in cancer (Hec1) in human bladder cancer and its relationship with clinicopathological factors and prognosis.Methods Immunohistochemistry and Western blot were used to detect the expression of Hec1 in 105 cases of surgically resected human bladder cancer and 20 para-carcinoma normal bladder histopathological specimens. Clinical TNM stage Ⅰ included 31 cases of metastasis and 34 cases without lymph node metastasis. Tumor diameter was 2 cm or less in 49 patients, and 2-5 cm in 35 cases. Twenty-three cases were in stage Ⅱ and 51 cases in stages Ⅲ+Ⅳ. There were 71 cases with lymph node metastasis, and tumor diameter was above 5 cm in 21 patients. The relationship between Hec1, clinicopathological factors and 5-year survival was analyzed by Prism6.0. The Cox proportional hazard model was used to analyze the independent prognostic factors of bladder cancer.Results Hec1 was significantly higher in human bladder cancer than that in para-cancer normal tissue (P<0.01). Hec1 was not significantly correlated with age, the histological type or tumor size(P values were 0.25, 0.17 and 0.26, respectively). However, the level of Hec1 increased with the clinical staging (P=0.042) and lymph node metastasis (P=0.033). The survival rate of Caveolin-1 in bladder cancer patients was lower than that of Hec1 patients (P=0.025). Lymph node metastasis, TNM stage and Hec1 were independent prognostic factors for bladder cancer.Conclusions Hec1 may play the role of a tumor stimulating factor and can facilitate clinical diagnosis, treatment and prognosis.
苏宏, 宋博, 张珊珊, 李青, 于新路, 王雪, 高万峰. Hec1在膀胱癌组织中的表达及其与临床病理因素和预后的关系[J]. 武警医学, 2019, 30(7): 560-563.
YUE Shuxiang, LIAN Yufei, LIU Guangshu. Expression of Hec-1 in bladder cancer and its clinical significance. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(7): 560-563.
McAinsh A D, Tytell J D, Sorger P K, et al. slctIIre, function, and regulation of budding yeast kinetochores [J]. Annu Rev Cell Dev Biol, 2003, 19: 519-539.
[2]
回允中(译). 阿克曼外科病理学[M]. 北京:人民卫生出版社,2009:1603-1615.
[3]
National comprehensive cancer network. NCCN clinical practice guidelines in oncology: breast screening and diagnosis, version.1.2010[M]. Washington: National Comprehensive Cancer Network, Inc, 2009: 3.
[4]
Cleveland D W, Mao Y, Sullivan K F. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling [J]. Cell, 2003, 112(4): 407-421.
[5]
Hori T, Haraguchi T, Hiraoka Y, et al. Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in verte-brate cells[J]. J Cell Sci, 2015, 116(16): 3347-3362.
[6]
Strunnikov A V, Hogan E, Koshland D. SMC2, a saccharomyces cerevisiae gene essential for chromosomesegregation and condensation, defines a subgroup within the SMC family [J]. Genes Dev, 2002, 9(5): 587-599.
[7]
Dehca J G, Dong Y, Hergert P. Hec1 and Nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites [J]. Mol Biol Cell, 2005, 16(2): 519-531.
[8]
Chen Y, Riley D J, Chen P L, et al. HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis[J]. Molec Cell Biol, 2014, 17(10):6049-6056.
[9]
Lanni J S, Jacks T. Characterization of the p53-dependent postmitotic checkpoint following spindle disruption [J]. Mol Cell Biol, 2011, 18(2):1055-1064.
[10]
Dobles M. Liberal V. Scott M L. Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2 [J]. Cell, 2000, 101(6):635-645.
[11]
McCleland M L, Gardner R D, Kallio M J, et al. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity [J]. Genes Dev, 2003, 17(1): 101-114.
[12]
Abrieu A, Magnaghi-Jaulin L, Kahana J A, et al. Mpsl is a kinetochore-associated kinase essential for the vertebrate mitotic checkpoint [J]. Cell, 2001, 106(1): 83-93.
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