Determination of resveratrol and ε-viniferincontents in the stems of grape vines from different habitats
SONG Fei1, HE Lingshuang2, SUN Huageng2, ZHAO Yanmin2,3, LIU Dan4, FU Shan2, LIU Dailin3
1.Meidical and Research Department,Characteristic Medical Center of PAP, Tianjin 300162 , China; 2.School of Chinese Meteria Medic,Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; 3.Pharmaceutical Department,Logistics University of PAP ,Tianjin 300309 , China; 4.Tianjin Jian Feng Natural Product R&D Co.,Ltd, Tianjin 300457, China
Abstract：Objective To determine the contents of resveratrol and ε-viniferin in the stems of muscat grape vines grown in different areas.Methods High performance liquid chromatography was applied to determine the contents with a mobile phase that consisted of methanol-acetic acid aqueous solution by gradient elution. The wavelength of detection was 310 nm and 254 nm respectively, the flow rate was 1 mL/min, and the column temperature was 30 ℃. Results The linear relationships of resveratrol and ε-viniferin were good in the range of 1.76 to 17.6 μg and 2.75 to 27.54 μg respectively(R2=0.9999). The contents of resveratrol and ε-viniferin determined with this method ranged from 1.94 to 5.96μg/g and 8.74 to 15.41 μg/g in ten samples, while their coefficients of variation were 40.64% and 17.67%, respectively.Conclusions This method is simple, accurate and can be used to for quality control of grape vines.
宋菲, 贺凌霜, 孙华庚, 赵艳敏, 刘丹, 付山, 刘岱琳. 不同产地葡萄藤茎中白藜芦醇和ε-葡萄素的含量测定[J]. 武警医学, 2019, 30(7): 599-602.
REN Yongqiang, WEI Yajuan, CAO Yanan, HE Guanglun, LIU Hailing. Determination of resveratrol and ε-viniferincontents in the stems of grape vines from different habitats. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(7): 599-602.
Yao Ch S, Huang K S, Lin M, et al .A new stilbene dimer from vitisamurensis[J]. J Asian Nat Prod Res, 2013, 15(6): 693-695.
[5]
Chou W F, Shen C C.Oligostilbenes from the roots of vitisthunbergii[J]. Planta Med. 2009, 75: 856-859.
[6]
Huang K S, Lin M, Yu L N,et al.Four novel oligostilbenes from the roots of vitisamurensis[J]. Tetrahedron, 2000, 56:1321-1329.
[7]
Nahla Z,Shigeki Y,Masahiro A, et al. Greater effectiveness of ε-viniferin in red wine than its monomer resveratrol for inhibiting vascular smooth muscle cell proliferation and migration[J]. Biosci Biotechnol Biochem, 2011,75(7): 1259-1267.
Liu M H,Lin X L,Li J,et al.Resveratrol induces apoptosis through modulation of the Akt / FoxO3a / Bim pathway in HepG2 cells[J].Mol Med Rep,2016,13: 1689-1694.
[15]
Nikhil K,Sharan S,Singh AK,et al.Anticancer activities of pterostilbene-isothiocyanate conjugate in breast cancer cells: involvement of PPARγ. [J].PLoS One,2014,9:e104592.
2019, Vol. 30 
