Abstract：Objective To explore the mechanism by which microRNA-26b-5p (miR-26b-5p) mediates high-mobility group A1 (HMGA1) in epithelial-mesenchymal transformation of ovarian cancer.Methods The target gene of microRNA-26b-5p was predicted. The expressions of miR-26b-5p and HMGA1 in tissues were detected by real-time reverse transcription PCR (qRT-PCR). After grouping and transfection of ovarian cancer cells, the expressions of mRNA and protein of relevant factors were detected by qRT-PCR and Western blot in the cells. Transwell invasion test was used to detect cell invasion and scratch test to detect migration ability, while qRT-PCR and Western blot were used to detect the expressions of E-cadherin and Vimentin genes related to epithelial-mesenchymal transformation in tumors.Results The expression of miR-26b-5p in ovarian cancer tissue decreased, while the expression of HMGA1 increased (All P＜0.05). miR-26b-5p mimic and sh-HMGA1 groups showed decreased expressions of HMGA1 and Vimentin, increased expression of E-cadherin, and reduced cell invasion and migration (All P＜0.05). Co-transfection of miR-26b-5p inhibitor and sh-HMGA1 reversed the inhibitory effect of sh-HMGA1 on epithelial-mesenchymal transformation (All P＜0.05).Conclusions Over-expression of miR-26b-5p inhibits the expressions of HMGA1 and Vimentin in ovarian cancer cells, promotes E-cadherin expression, reduces cell invasion and migration, thus inhibiting epithelial-mesenchymal transformation of ovarian cancer cells.
曹文红. 过表达miR-26b-5p抑制HMGA1参与卵巢癌细胞上皮间质转化的机制[J]. 武警医学, 2019, 30(9): 760-763.
CAO Wenhong. Over-expression of MiR-26b-5p inhibits HMGA1 expression and participates in epithelial-mesenchymal transformation of ovarian cancer cells. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(9): 760-763.
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2019, Vol. 30 
