Elevated serum and urinary miRNA- 150 and miRNA-30a-5P levels in children with idiopathic nephrotic syndrome
SHI Zhongrui1, LI Weixin1, ZHANG Minghua2
1. Department of Clinical Laboratory,Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin 300162, China; 2. Logistics University of Chinese People’s Armed Police Force, Tianjin 300300, China
Abstract：Objective To analyze the difference of expressions of miRNA-150 and miRNA-30a-5P in serum and urine of children with idiopathic nephrotic syndrome (INS)and discuss their clinical value as a non-invasive auxiliary diagnostic marker of INS.Methods Urine samples were collected from 93 INS children (the patient group) who were diagnosed but waited to be treated in our hospital between January 2017 and December 2019 and from another 89 sex- and age-matched healthy children (the control group). The levels of serum and urinary miRNA-150 and miRNA-30a-5P were determined by quantitative real-time polymerase chain reaction (qRT-PCR) . The clinical value of serum and urinary miRNA-150 and miRNA-30a-5P in the diagnosis of children’s INS was assessed by the ROC curve.Results The levels of serum and urinary miRNA-30a-5p and serum miRNA-150 were slightly higher in the patient group than in healthy controls (P<0.05) , but the levels of urinary miRNA-150 were not. The levels of serum and urinary miRNA-30a-5P and serum miRNA-150 in the clinical remission period decreased significantly in forty-six of these cases(P<0.05) .The area under the ROC curve (AUCROC) of serum miRNA-150 and miRNA-30a-5P was 0.852 and 0.869 respectively for the diagnosis of INS children, compared to 0.825 and 0.833 for urinary miRNA-150 and miRNA-30a-5P.Conclusions The serum and urinary levels of miRNA-150 and miRNA-30a-5P in children with idiopathic nephrotic syndrome increase obviously, which may serve as new biopsy molecular markers for the auxiliary diagnosis of INS.
师钟睿, 李伟鑫, 张明华. 血清、尿液中miRNA-150及miRNA-30a-5P对儿童特发性肾病综合征的诊断价值[J]. 武警医学, 2020, 31(8): 695-698.
SHI Zhongrui, LI Weixin, ZHANG Minghua. Elevated serum and urinary miRNA- 150 and miRNA-30a-5P levels in children with idiopathic nephrotic syndrome. Med. J. Chin. Peop. Armed Poli. Forc., 2020, 31(8): 695-698.
Deschênes G, Dossier C, Hogan J.Treating the idiopathic nephrotic syndrome: are steroids the answer?[J].Pediatr Nephro, 2019,34(5):777-785.
[5]
Kemper M J, Valentin L, Husen M.Difficult to treat idiopathic nephrotic syndrome: established drugs, open questions and future options[J]. Pediatr Nephro, 2018,33(10):1641-1649.
[6]
Parvex, Paloma.Are micro RNA potential biomarkers in children with idiopathic nephrotic syndrome? [J]. EBio Medicine, 2019,39:27-28.
[7]
Kang H G,Seo H, Lim J H, et al. Markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome: whole-transcriptome sequencing of peripheral blood mononuclear cells[J]. JMR, 2017,45(3):948-963.
[8]
Chen T, Wang C, Yu H, et al. Increased urinary exosomal micro RNAs in children with idiopathicnephrotic syndrome[J].EBio Medicine,2019, 39:552-561.
[9]
Hu S, Han R, Shi J,et al. The long noncoding RNA LOC105374325 causes podocyte injury in individuals with focal segmental glomerulosclerosis [J]. JBC, 2018,293 (52):20227-20239.
[10]
Yang R, Hong H, Wang M,et al. Correlation between single-nucleotide polymorphisms within mir-30a and related target genes and risk or prognosis of nephrotic syndrome [J]. DNA Cell Biol, 2018,37(3):233-243.
Kang H G,Seo H, Lim J H,et al. Markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome: whole transcriptome sequencing of peripheral blood mononuclear cells [J]. JMR,2017,45(3):948-963.
[13]
Chen T, Wang C, Yu H,et al.Increased urinary exosomal microRNAs in children with idiopathic nephrotic syndrome[J].EBio Medicine,2019,39:552-561.
[14]
Wang Y, Zheng C, Wang X, et al. Proteomic profile based screening of potential protein biomarkers in the urine of patients with nephrotic syndrome [J]. Mol Med Report, 2017,16(5):6276-6284.
[15]
Patnaik S K, Kumar P, Yadav P,et al.Can micro RNA profiles predict corticosteroid responsiveness in childhood nephrotic syndrome a study protocol [J]. BMJ,2018,2(1):e000319.
[16]
Li J, Donath S, Li Y,et al.miR-30 regulates mitochondrial fission through targeting p53 and the dynamin-related protein-1 pathway [J]. PLoS Genet,2010,6(1):e1000795.
[17]
Fan P C, Chen C C, Peng C C,et al .A circulating miRNA signature for early diagnosis of acute kidney injury following acute myocardial infarction[J]. J Transl Med,2019,17(1):139.
2020, Vol. 31 
