HPLC法同时测定参龙通络丸中三七皂苷R<sub>1</sub>和人参皂苷Rf的含量

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (705 KB)
输出: BibTeX | EndNote (RIS)

摘要目的建立一种可以同时测定参龙通络丸中两种成分(三七皂苷R₁、人参皂苷Rf)含量的HPLC法。方法采用高效液相色谱法(HPLC),Hypersil Hypersil BDS C₁₈色谱柱(5 μm,4 mm×250 mm),乙腈-水为流动相梯度洗脱;流速1.0 ml/min;检测波长203 nm;柱温35 ℃,检测参龙通络丸中两种成分的含量。结果三七皂苷R₁样品量在0.198~9.535 μg范围(r=0.9993)、人参皂苷Rf的样品量在0.957~38.392 μg范围(r=0.9997)线性关系良好,两种成分的平均回收率分别为99.10%(RSD=1.57％,n=6)和98.76% (RSD=2.13％,n=6)。结论本方法具有结果准确、可靠、重复性好的特点,可为参龙通络丸的质量标准制定提供科学依据。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	杨媛媛
	栾涛
	孙玉坤
	郭兆刚

关键词 ：参龙通络丸, 三七皂苷R₁, 人参皂苷Rf, 高效液相色谱法

Abstract：Objective To establish an HPLC method for simultaneous determination of Notoginsenoside R₁ and ginsenoside Rf in Shenlong Tongluo pills.Methods High performance liquid chromatography was used.Hypersil Hypersil BDS C₁₈(5 μm,4 mm×250 mm) column was selected with acetonitrile-water as the mobile phase for gradient elution.The flow rate was 1.0 ml/min,detection wavelength was 203 nm and the column temperature was set at 35 ℃.Results The linear ranges of Notoginsenoside R₁ and ginsenoside Rf were 0.198-9.535 μg (r=0.9993) and 0.957-38.392 μg(r=0.9997) respectively.The average recoveries were 99.10％(RSD=1.57％,n=6) and 98.76％(RSD=2.13％,n=6)respectively.Conclusions This HPLC method was accurate,reliable and reproducible,which can contribute to formulating the quality standard of Shenlong Tongluo pills.

Key words： Shenlong Tongluo pills Notoginsenoside R₁ ginsenoside Rf HPLC

收稿日期: 2020-09-20

ZTFLH:

R284.1

通讯作者: 郭兆刚,E-mail:wjsunyk@163.com

作者简介: 杨媛媛,本科学历,副主任药师。

引用本文:

杨媛媛, 栾涛, 孙玉坤, 郭兆刚. HPLC法同时测定参龙通络丸中三七皂苷R₁和人参皂苷Rf的含量[J]. 武警医学, 2021, 32(1): 15-18. YANG Yuanyuan, LUAN Tao, SUN Yukun, GUO Zhaogang. Simultaneous determination of Notoginsenoside R₁ and ginsenoside Rf in Shenlong Tongluo pills by HPLC. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(1): 15-18.

链接本文:

http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2021/V32/I1/15

[1]	王晶,张勇,毕萌菲,等.RP-HPLC法分析测定人参、西洋参、三七中的皂苷类化合物[J].食品研究与开发,2020,41(7):172-176.
[2]	Wang Y,Choi H k,Brincmann J A,et al.Chemical analysis of Panax quinquefolius(North American ginseng):a review[J].J Chroma A,2015,1426:1-15.
[3]	Zhang X,Gao Y,Zang P,et al.Study on the simultaneous degradation of five pesticides by Paenibacillus polymyxa from Panax ginseng and the characteristics of their products[J].Ecotoxicology and Environmental Safety,2019,168:415-422.
[4]	何耀慧,林荣锋,陈剑平,等.人参、三七在复方芪术调经散中的高效液相色谱法鉴别[J].广州中医药大学学报,2009,26(5):468-470.
[5]	杨雨.西洋参茎叶皂苷类化学成分的研究[D].北京:中国农业科学院,2014.
[6]	董晓强,董文天,洪霞,等.三七、人参和西洋参化学成分与药效学之间的关系[J].承德医学院学报,2011,28(3):307-309.
[7]	陈剑平,郑平,李中桂,等.HPLC-MS法同时测定健脾益肾丸中8个成分的含量[J].药物分析杂志,2017,37(6):962-967.
[8]	张永,陈旭,丁越,等.HPLC法结合统计学方法研究复方丹参片中皂苷类成分[J].辽宁中医杂志,2019,46(1):120-123.
[9]	靳瑞婷,杨素德,付娟,等.一测多评法测定芪白平肺颗粒中8种皂苷类成分[J].中草药,2015,46(24):3682-3686.
[10]	Zhang Jing,Chen Quancheng,Gong Xiaojie,et al.Effect of different method on extracting ratios of ginsenosides[J].J Jilin Agricul University,2003,25(1):71-73.
[11]	宋幸辉,郑宝亮,王长林,等,人参茎叶总皂苷的提取方法研究[J].现代牧业,2017,1(1):30-32.
[12]	翟为民,袁永生,周玉新,等.人参、西洋参及三七参指纹图谱鉴别[J].中国中药杂志,2001,26(7):481.
[13]	张文宗,张璞.HPLC波长切换法同时测定益安宁丸中7种活性成分[J].中草药,2019,50(8):1947-1951.
[14]	李宁,高小惠,王子幼,等.三七HPLC指纹图谱及5种成分测定[J].中成药,2020,42(5):1233-1237.
[15]	郭冲,郜玉钢,臧埔,等.HPLC法同时测定人参及其制剂中16种人参皂苷[J].中草药,2014,45(14):2009-2013.