Protective effect of suberoylanilide hydroxamic acid on heart of rats with severe scald injury
MENG Xiangxi1, WEN Hailing1, ZHENG Jinguang2, HU Sen3
1. Department of Burn and Plastic Surgery, Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China, 2. Burn Research Institute, 3. Research Laboratory of Shock and Multiple Organ Dysfunction, Forth Medical Center of PLA General Hospital, Beijing 100048,China
Abstract：Objective To study the protective effect of suberoylanilide hydroxamic acid(SAHA) on the heart of rats with severe scald injury.Methods Forty-eight male SD rats were randomly divided into the sham scald group, the scald group and the SAHA group (n=16). Rats in the scald group and SAHA group were subjected to 50％TBSA third-degree full-thickness scald injury with boiled water before they received an intraperitoneal injection of 0.25 ml of 0.9% normal saline(NS)or SAHA (7.5 mg/kg, dissolved in 0.25 ml of NS) . 37 ℃ water instead of boiled water was used for rats in the sham scald group. According to time points for observation, each scald group was divided into two subgroups: the 3-hour group and 6-hour group after scald(n=8). At 3h or 6h after burns, blood in each group was withdrawn from the abdominal aorta to detect the level of CK-MB before the rats were sacrificed. Myocardial tissues were harvested, the water content of which was detected by dry/wet weight method. The rate of myocardial apoptosis in each group was calculated using the TUNEL method while the activity of caspase-3 was measured by detection kits.Results At 3 h and 6 h after scald, the level of CK-MB in the scald group[(2552.9±145.3)U/L,(5418.7±292.9)U/L] was more significantly increased than in the sham scald group[(585.6±46.1)U/L], so were the water content of myocardial tissues [(77.65±1.75)%,(80.23±1.82)%]vs.[(70.12±1.99)%], rate of myocardial apoptosis [(16.1±0.8)%,(21.5±1.2)%] vs. [(0.5±0.1)%], and the activity of caspase-3 [(0.716±0.052)μmol/mg;(0.912±0.063)μmol/mg] vs. [(0.435±0.041)μmol/mg], and the difference was statistically significant (all P<0.05). After SAHA treatment, the level of CK-MB [(1642.4±157.2) U/L, (3475.3±187.5) U/L], water content of myocardial tissues [(72.31±1.95)%, (75.21±2.00)%],rate of myocardial apoptosis[(11.6±0.6)%,(15.1±0.9)%],and activity of caspase-3[(0.557±0.052)μmol/mg,(0.535±0.034)μmol/mg]were significantly lower than in the scald group.Conclusions In rats with fatal scald, SAHA can reduce the level of CK-MB in plasma and the water content of myocardial tissues while protecting cardiac function,which may be related to the decrease of the apoptosis of myocardial cells and the activity of caspase-3 in myocardium.
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2021, Vol. 32 
